Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir

doi:10.1007/s00203-016-1284-7

	Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir
	Zhen, Zhuo 1,2; Liu, Jingwen 3; Rensing, Christopher 1,4; Yan, Changzhou 1; Zhang, Yongyu1,5
	2017
发表期刊	ARCHIVES OF MICROBIOLOGY
卷号	199 期号:1 页码:125-134
摘要	The use of molecular methods to investigate the community structure and diversity of microalgae has largely replaced the previous morphological methods that were routinely carried out by microscopy. Different DNA polymerases can lead to bias in PCR amplification and affect the downstream community and diversity analysis. In this study, two clone libraries were constructed with two different DNA polymerases, Q5 high-fidelity DNA polymerase and exTaq polymerase, to compare the differences in their capability to accurately reflect the cyanobacterial community structure and diversity in a subtropical deep freshwater reservoir, Dongzhen reservoir. The results indicated that the two cyanobacterial clone libraries constructed by using Q5 high-fidelity DNA polymerase and exTaq DNA polymerase did not show significant differences, although a slightly higher community diversity was revealed by using Q5 high-fidelity DNA polymerase. It is noteworthy that so far Q5 high-fidelity DNA polymerase was the first time to be employed in the genetic analysis of cyanobacterial community. And it is for the first time that the cyanobacterial community structure in Dongzhen reservoir was analyzed using molecular methods. Phylogenetic analysis revealed that most of the clones belonged to Cyanophyta and chloroplast, among which Cyanobium sp. Suigetsu-CR5 made up the largest fraction of cyanobacteria in winter.
文章类型	Article
关键词	Cyanobacterial Diversity Clone Library Q5 Dna Polymerase Extaq Polymerase
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00203-016-1284-7
关键词[WOS]	CYLINDROSPERMOPSIS-RACIBORSKII ; EVOLUTIONARY ANALYSIS ; LYNGBYA-MAJUSCULA ; MICROBIAL MATS ; DIVERSITY ; BACTERIAL ; SEQUENCES ; ZONES ; BLOOM
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	Shandong Provincial Key Laboratory of Energy Genetics(SDKLEG201508) ; National Natural Science Foundation of China(21577138 ; 41576166)
WOS类目	Microbiology
WOS记录号	WOS:000392121800016
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9181
专题	海洋碳汇与能源微生物研究组
作者单位	1.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.JiMei Univ, Coll Food & Bioengn, Xiamen 361021, Peoples R China 4.Univ Copenhagen, Dept Plant & Environm Sci, Frederiksberg, Denmark 5.Chinese Acad Sci, Res Ctr Marine Biol & Carbon Sequestrat, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Zhen, Zhuo,Liu, Jingwen,Rensing, Christopher,et al. Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir[J]. ARCHIVES OF MICROBIOLOGY,2017,199(1):125-134.
APA	Zhen, Zhuo,Liu, Jingwen,Rensing, Christopher,Yan, Changzhou,&Zhang, Yongyu.(2017).Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir.ARCHIVES OF MICROBIOLOGY,199(1),125-134.
MLA	Zhen, Zhuo,et al."Effects of two different high-fidelity DNA polymerases on genetic analysis of the cyanobacterial community structure in a subtropical deep freshwater reservoir".ARCHIVES OF MICROBIOLOGY 199.1(2017):125-134.