| Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth | |
| Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu; Wei Du | |
| 4296 | |
| Source Publication | Sensors and Actuators B-Chemical
 |
| Volume | 255Pages:735-744 |
| Contribution Rank | 2 |
| Abstract | C. elegans is a leading model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient-customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL-1 and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen. |
| Subtype | Article |
| DOI | 10.1016/j.snb.2017.08.088 |
| WOS Keyword | CAENORHABDITIS-ELEGANS ; MICROFLUIDIC DEVICE ; QUANTITATIVE-ANALYSIS ; DEVELOPMENTAL-STAGES ; LIFE-SPAN ; GENETICS ; PLATFORM ; CHEMOTAXIS ; LONGEVITY ; PATHWAYS |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| Funding Organization | National Natural Science Foundation of China ; National Key R&D Program of China |
| WOS Subject | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS ID | WOS:000414151800088 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qibebt.ac.cn/handle/337004/9869 |
| Collection | 单细胞中心组群 |
| Corresponding Author | Wei Du |
| Recommended Citation GB/T 7714 | Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,Wei Du. Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth[J]. Sensors and Actuators B-Chemical,4296,255:735-744. |
| APA | Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,&Wei Du.(4296).Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth.Sensors and Actuators B-Chemical,255,735-744. |
| MLA | Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth".Sensors and Actuators B-Chemical 255(4296):735-744. |
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| Logarithmic bacteria（2311KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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