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Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth
Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu; Wei Du
4296
Source PublicationSensors and Actuators B-Chemical
Volume255Pages:735-744
Contribution Rank2
AbstractC. elegans is a leading model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient-customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL-1 and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.
SubtypeArticle
DOI10.1016/j.snb.2017.08.088
WOS KeywordCAENORHABDITIS-ELEGANS ;  MICROFLUIDIC DEVICE ;  QUANTITATIVE-ANALYSIS ;  DEVELOPMENTAL-STAGES ;  LIFE-SPAN ;  GENETICS ;  PLATFORM ;  CHEMOTAXIS ;  LONGEVITY ;  PATHWAYS
Indexed BySCI
Language英语
WOS Research AreaChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
Funding OrganizationNational Natural Science Foundation of China ; National Key R&D Program of China
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000414151800088
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9869
Collection单细胞中心组群
Corresponding AuthorWei Du
Recommended Citation
GB/T 7714
Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,Wei Du. Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth[J]. Sensors and Actuators B-Chemical,4296,255:735-744.
APA Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,&Wei Du.(4296).Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth.Sensors and Actuators B-Chemical,255,735-744.
MLA Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth".Sensors and Actuators B-Chemical 255(4296):735-744.
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