Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death

doi:10.3389/fpls.2017.00982

QIBEBT-IR > 能源作物分子育种研究组

	Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death
	Liu, Sijia 1,2; Fu, Chunxiang3 ; Gou, Jiqing 2,4; Sun, Liang 5; Huhman, David 6; Zhang, Yunwei 1; Wang, Zeng-Yu 2,4
	2017-06-20
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	8
摘要	Switchgrass (Panicum virgatum) has been developed into a model lignocellulosic bioenergy crop. Downregulation of caffeic acid O-methyltransferase (COMT), a key enzyme in lignin biosynthesis, has been shown to alter lignification and increase biofuel yield in switchgrass. Methylenetetrahydrofolate reductase (MTHFR) mediates C1 metabolism and provides methyl units consumed by COMT. It was predicted that co-silencing of MTHFR and COMT would impact lignification even more than either of the single genes. However, our results showed that strong downregulation of MTHFR in a COMT-deficient background led to altered plant growth and development, but no significant change in lignin content or composition was found when compared with COMT plants. Another unexpected finding was that the double MTHFR/COMT downregulated plants showed a novel lesion-mimic leaf phenotype. Molecular analyses revealed that the lesion-mimic phenotype was caused by the synergistic effect of MTHFR and COMT genes, with MTHFR playing a predominant role. Microarray analysis showed significant induction of genes related to oxidative and defense responses. The results demonstrated the lack of additive effects of MTHFR and COMT on lignification. Furthermore, this research revealed an unexpected role of the two genes in the modulation of lesion-mimic cell death as well as their synergistic effects on agronomic performance.
文章类型	Article
关键词	Bioenergy Crop Caffeic Acid O-methyltransferase (Comt) Lesion-mimic Cell Death Lignin Methylenetetrahydrofolate Reductase (Mthfr) Panicum Virgatum Switchgrass Transgenic Plants
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fpls.2017.00982
关键词[WOS]	ACID O-METHYLTRANSFERASE ; BROWN-MIDRIB MUTANTS ; MODIFIED TOBACCO REVEALS ; METHYLENETETRAHYDROFOLATE REDUCTASE ; LIGNIN BIOSYNTHESIS ; PRIMARY METABOLISM ; DEFENSE RESPONSE ; FIELD ANALYSIS ; GENE ENCODES ; MAIZE
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
项目资助者	China Scholarship Council ; BioEnergy Science Center ; Samuel Roberts Noble Foundation ; Ministry of Science and Technology, PR China(2014BAD23B03-02 ; 2012AA101801)
WOS类目	Plant Sciences
WOS记录号	WOS:000403638700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9712
专题	能源作物分子育种研究组
作者单位	1.China Agr Univ, Dept Grassland Sci, Natl Energy R&D Ctr Biomass, Beijing, Peoples R China 2.Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 4.US DOE, Oak Ridge Natl Lab, BioEnergy Sci Ctr, Oak Ridge, TN 37830 USA 5.Samuel Roberts Noble Fdn Inc, Comp Serv, Ardmore, OK USA 6.Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK USA
推荐引用方式 GB/T 7714	Liu, Sijia,Fu, Chunxiang,Gou, Jiqing,et al. Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Liu, Sijia.,Fu, Chunxiang.,Gou, Jiqing.,Sun, Liang.,Huhman, David.,...&Wang, Zeng-Yu.(2017).Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death.FRONTIERS IN PLANT SCIENCE,8.
MLA	Liu, Sijia,et al."Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death".FRONTIERS IN PLANT SCIENCE 8(2017).