| Structural Insight into the Stabilizing Effect of O-Glycosylation | |
Chaffey, Patrick K.1,2; Guan, Xiaoyang1,2; Chen, Chao3,4 ; Ruan, Yuan1,2; Wang, Xinfeng1,2; Tran, Amy H.1,2; Koelsch, Theo N.1,2; Cui, Qiu3,4; Feng, Yingang3,4; Tan, Zhongping1,2
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| 2017-06-13 | |
| 发表期刊 | BIOCHEMISTRY
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| 卷号 | 56期号:23页码:2897-2906 |
| 摘要 | Protein glycosylation has been shown to have a variety of site specific and glycan-specific effects, but so far, the molecular logic that lead's to such observations has been elusive. Understanding the structural changes that Occur and being able to correlate those with the physical properties of the glycopeptide are valuable steps toward being able to predict how specific glycosylation patterns will affect the stability of glycoproteins: By systematically comparing the structural features of the O-glycosylated carbohydrate-binding module of a Trichoderma reesei-derived Family 7 cellobiohydrolase, we were able to develop a better understanding of the influence of O-glycan structure on the molecule's physical stability. Our results indicate that the previously observed stabilizing effects of O-glycans come from the introduction of new bonding interactions to the structure and increased rigidity, while the decreased stability seemed to result from the impaired interactions and increased conformational flexibility. This type of knowledge provides a powerful and potentially general mechanism for improving the stability of proteins through glycoengineering. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1021/acs.biochem.7b00195 |
| 关键词[WOS] | CARBOHYDRATE-BINDING MODULE ; N-LINKED GLYCANS ; CHEMOENZYMATIC SYNTHESIS ; CHEMICAL GLYCOBIOLOGY ; PROTEIN STRUCTURES ; BIOLOGICAL ROLES ; NMR ANALYSIS ; DYNAMICS ; GLYCOPEPTIDE ; RELAXATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 项目资助者 | University of Colorado Boulder ; National Science Foundation CAREER Award(CHE-1454925) ; National Natural Science Foundation of China(31670735 ; Chinese Government Scholarship(201604910035) ; 31661143023) |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000403529900005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9708 |
| 专题 | 代谢物组学研究组 |
| 作者单位 | 1.Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA 2.Univ Colorado, BioFrontiers Inst, Boulder, CO 80303 USA 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chaffey, Patrick K.,Guan, Xiaoyang,Chen, Chao,et al. Structural Insight into the Stabilizing Effect of O-Glycosylation[J]. BIOCHEMISTRY,2017,56(23):2897-2906. |
| APA | Chaffey, Patrick K..,Guan, Xiaoyang.,Chen, Chao.,Ruan, Yuan.,Wang, Xinfeng.,...&Tan, Zhongping.(2017).Structural Insight into the Stabilizing Effect of O-Glycosylation.BIOCHEMISTRY,56(23),2897-2906. |
| MLA | Chaffey, Patrick K.,et al."Structural Insight into the Stabilizing Effect of O-Glycosylation".BIOCHEMISTRY 56.23(2017):2897-2906. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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