| Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides | |
| Abu Saleh, Mohammad1,2,3; Han, Wen-Jie1,2,4; Lu, Ming1,2; Wang, Bing1,2; Li, Huayue5; Kelly, Robert M.6; Li, Fu-Li1,2 | |
| 2017-07-01 | |
| 发表期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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| 卷号 | 83期号:13 |
| 摘要 | Species in the extremely thermophilic genus Caldicellulosiruptor can degrade unpretreated plant biomass through the action of multimodular glycoside hydrolases. To date, most focus with these bacteria has been on hydrolysis of glucans and xylans, while the biodegradation mechanism for arabinose-based polysaccharides remains unclear. Here, putative alpha-L-arabinofuranosidases (AbFs) were identified in Caldicellulosiruptor species by homology to less-thermophilic versions of these enzymes. From this screen, an extracellular XynF was determined to be a key factor in hydrolyzing alpha-1,2-, alpha-1,3-, and alpha-1,5-L-arabinofuranosyl residues of arabinose-based polysaccharides. Combined with a GH11 xylanase (XynA), XynF increased arabinoxylan hydrolysis more than 6-fold compared to the level seen with XynA alone, likely the result of XynF removing arabinofuranosyl side chains to generate linear xylans that were readily degraded. A second AbF, the intracellular AbF51, preferentially cleaved the alpha-1,5-L-arabinofuranosyl glycoside bonds within sugar beet arabinan. beta-Xylosidases, such as GH39 Xyl39B, facilitated the hydrolysis of arabinofuranosyl residues at the nonreducing terminus of the arabinose-branched xylo-oligosaccharides by AbF51. These results demonstrate the separate but complementary contributions of extracellular XynF and cytosolic AbF51 in processing the bioconversion of arabinose-containing oligosaccharides to fer-mentable monosaccharides. |
| 文章类型 | Article |
| 关键词 | Arabinofuranosidase Bioenergy Glycoside Hydrolase Hyperthermophiles Synergism |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1128/AEM.00574-17 |
| 关键词[WOS] | FREE QUANTITATIVE PROTEOMICS ; GLYCOSIDE HYDROLASE ; PLANT BIOMASS ; INTESTINAL SUCRASE ; GH11 XYLANASES ; DSM 6725 ; SP F32 ; SPECIFICITY ; DIVERSITY ; CELLULOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 项目资助者 | National Natural Science Foundation of China(31400060) ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; Key Scientific and Technological Project of Shandong Province(2015ZDXX0403A01) |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000403495700015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/9640 |
| 专题 | 分子微生物工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao Engn Lab Single Cell Oil,Key Lab Biofuel, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Rajshahi, Dept Genet Engn & Biotechnol, Rajshahi, Bangladesh 4.Qingdao Univ, Coll Environm Sci & Engn, Qingdao, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China 6.North Carolina State Univ, Dept Chem & Biomol Engn, Raleigh, NC USA |
| 推荐引用方式 GB/T 7714 | Abu Saleh, Mohammad,Han, Wen-Jie,Lu, Ming,et al. Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(13). |
| APA | Abu Saleh, Mohammad.,Han, Wen-Jie.,Lu, Ming.,Wang, Bing.,Li, Huayue.,...&Li, Fu-Li.(2017).Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(13). |
| MLA | Abu Saleh, Mohammad,et al."Two Distinct alpha-L-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.13(2017). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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