Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation

doi:10.1007/s00253-016-7949-y

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	Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation
	Zhang, Xiaomei 1; Hua, Xiufu 2; Yue, Xiuping 3
	2017-02-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	101 期号:4 页码:1697-1707
摘要	For quinoline-denitrifying degradation, very few researches focused on shortcut denitrification process and its bacterial community characteristics. In this study, complete and shortcut denitrification systems were constructed simultaneously for quinoline degradation. By calculation, specific quinoline removal rates were 0.905 and 1.123 g/(gVSS d), respectively, in the complete and shortcut systems, and the latter was 1.24 times of the former. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), high-throughput sequencing, and quantitative PCR (qPCR) techniques based on 16S rRNA were jointly applied to compare microbial community structures of two systems. Many denitrifying bacteria phyla, classes, and genera were detected in the two systems. Phylum Proteobacteria, Class Gammaproteobacteria, and Genus Alicycliphilus denitrificans were the dominant contributors for quinoline-denitrifying degradation. In the shortcut denitrification system, main and specific strains playing crucial roles were more; the species richness and the total abundance of functional genes (narG, nirS, nirK, and nosZ) were higher compared with the complete denitrification system. It could be supposed that inorganic-nitrogen reductase activity of bacterial community was stronger in the shortcut denitrification system, which was the intrinsic reason to result in higher denitrification rate.
文章类型	Article
关键词	Quinoline Degradation Shortcut Denitrification Bacterial Community Pcr-dgge High-throughput Sequencing Qpcr
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-016-7949-y
关键词[WOS]	RIBOSOMAL-RNA GENE ; WASTE-WATER ; MICROBIAL COMMUNITY ; FUNCTIONAL GENES ; HYDROGENOTROPHIC DENITRIFICATION ; CONSTRUCTED WETLAND ; DIVERSITY ; REACTOR ; POPULATIONS ; PCR
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China(51,378,330) ; State Key Joint Laboratory of Environment Simulation and Pollution Control(15K03ESPCT)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000393687400033
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9321
专题	规划战略与信息中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Tsinghua Univ, Dept Sci Res & Dev, Beijing 100084, Peoples R China 3.Taiyuan Univ Technol, Environm Sci & Engn Inst, 79 Yingze West St, Taiyuan 030024, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaomei,Hua, Xiufu,Yue, Xiuping. Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(4):1697-1707.
APA	Zhang, Xiaomei,Hua, Xiufu,&Yue, Xiuping.(2017).Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(4),1697-1707.
MLA	Zhang, Xiaomei,et al."Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.4(2017):1697-1707.