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A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans
Wang, Xixian1,2,3,4; Ge, Anle1,2; Hu, Liang1,2; Feng, Xiaojun1,2; Du, Wei1,2; Liu, Bi-Feng1,2
2017-06-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号244页码:1152-1159
摘要C. elegans was widely used as a prominent model organism in the study of physiological response to gaseous environment, thanks to its suitability for in vivo optical imaging of neuronal sensing. Immobilization of the active worms is a key procedure to obtain high-resolution imaging and gas stimulations. In this work, we presented a novel microfluidic approach for immobilizing C. elegans due to the fact that its movement could be eliminated under the condition of gentle surface-dehydration. Nitrogen gas was directly injected into the microfluidic chip to dehydrate the surface of worm appropriately, resulting in rapid and repetitive immobilization of C. elegans. The imaging analysis of subcellular distribution of DAF-16, a well-known transcription factor regulating different stress responses, indicated that this dehydration-based immobilization method would not induce obvious stress response of the worm within 20 min. Furthermore, this microfluidic device could generate gas stimulation to the immobilized worm without the interference resulted from the switch of liquid-gas. Finally, the developed micro-device clearly demonstrated that URX neuronal responses were induced by oxygen gas with increasing levels of 0-20%, indicating that this method could be used for imaging analysis of gas-evoked neuronal sensing, especially to the pressure-change sensitive neurons in C elegans. (C) 2017 Elsevier B.V. All rights reserved.
文章类型Article
关键词Microfluidic Chip C. Elegans Surface-dehydration Immobilization In Vivo Neuronal imagIng Gas Stimulation
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1016/j.snb.2017.01.083
关键词[WOS]NEMATODE CAENORHABDITIS-ELEGANS ; IN-VIVO ; DEVELOPMENTAL-STAGES ; MICROFLUIDIC DEVICE ; SYSTEM ; MICROINJECTION ; REGENERATION ; PLATFORM ; LIGHT ; ARRAY
收录类别SCI
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者National Basic Research Program of China(2011CB910403) ; National Natural Science Foundation of China(21475049 ; NIH National Centre for Research Resources ; 31471257)
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000395963300135
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/9301
专题单细胞中心组群
作者单位1.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect Hubei Bioinformat, Dept Biomed Engn,Coll Life Sci & Technol,Syst Bio, Wuhan 430074, Peoples R China
2.Huazhong Univ Sci & Technol, Mol Imaging Key Lab, Syst Biol Theme, Dept Biomed Engn,Coll Life Sci & Technol, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
4.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
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Wang, Xixian,Ge, Anle,Hu, Liang,et al. A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,244:1152-1159.
APA Wang, Xixian,Ge, Anle,Hu, Liang,Feng, Xiaojun,Du, Wei,&Liu, Bi-Feng.(2017).A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans.SENSORS AND ACTUATORS B-CHEMICAL,244,1152-1159.
MLA Wang, Xixian,et al."A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans".SENSORS AND ACTUATORS B-CHEMICAL 244(2017):1152-1159.
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