A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans

doi:10.1016/j.snb.2017.01.083

QIBEBT-IR > 单细胞中心组群

	A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans
	Wang, Xixian 1,2,3,4; Ge, Anle 1,2; Hu, Liang 1,2; Feng, Xiaojun 1,2; Du, Wei 1,2; Liu, Bi-Feng 1,2
	2017-06-01
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	244 页码:1152-1159
摘要	C. elegans was widely used as a prominent model organism in the study of physiological response to gaseous environment, thanks to its suitability for in vivo optical imaging of neuronal sensing. Immobilization of the active worms is a key procedure to obtain high-resolution imaging and gas stimulations. In this work, we presented a novel microfluidic approach for immobilizing C. elegans due to the fact that its movement could be eliminated under the condition of gentle surface-dehydration. Nitrogen gas was directly injected into the microfluidic chip to dehydrate the surface of worm appropriately, resulting in rapid and repetitive immobilization of C. elegans. The imaging analysis of subcellular distribution of DAF-16, a well-known transcription factor regulating different stress responses, indicated that this dehydration-based immobilization method would not induce obvious stress response of the worm within 20 min. Furthermore, this microfluidic device could generate gas stimulation to the immobilized worm without the interference resulted from the switch of liquid-gas. Finally, the developed micro-device clearly demonstrated that URX neuronal responses were induced by oxygen gas with increasing levels of 0-20%, indicating that this method could be used for imaging analysis of gas-evoked neuronal sensing, especially to the pressure-change sensitive neurons in C elegans. (C) 2017 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Microfluidic Chip C. Elegans Surface-dehydration Immobilization In Vivo Neuronal imagIng Gas Stimulation
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1016/j.snb.2017.01.083
关键词[WOS]	NEMATODE CAENORHABDITIS-ELEGANS ; IN-VIVO ; DEVELOPMENTAL-STAGES ; MICROFLUIDIC DEVICE ; SYSTEM ; MICROINJECTION ; REGENERATION ; PLATFORM ; LIGHT ; ARRAY
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者	National Basic Research Program of China(2011CB910403) ; National Natural Science Foundation of China(21475049 ; NIH National Centre for Research Resources ; 31471257)
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000395963300135
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9301
专题	单细胞中心组群
作者单位	1.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect Hubei Bioinformat, Dept Biomed Engn,Coll Life Sci & Technol,Syst Bio, Wuhan 430074, Peoples R China 2.Huazhong Univ Sci & Technol, Mol Imaging Key Lab, Syst Biol Theme, Dept Biomed Engn,Coll Life Sci & Technol, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 4.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xixian,Ge, Anle,Hu, Liang,et al. A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,244:1152-1159.
APA	Wang, Xixian,Ge, Anle,Hu, Liang,Feng, Xiaojun,Du, Wei,&Liu, Bi-Feng.(2017).A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans.SENSORS AND ACTUATORS B-CHEMICAL,244,1152-1159.
MLA	Wang, Xixian,et al."A novel on-chip immobilization strategy for imaging analysis of neuronal response to gas cues in C. elegans".SENSORS AND ACTUATORS B-CHEMICAL 244(2017):1152-1159.