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UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana
Lin, Ji-Shan1; Huang, Xu-Xu1; Li, Qin1; Cao, Yingping2; Bao, Yan2; Meng, Xia-Fei1; Li, Yan-Jie1; Fu, Chunxiang2; Hou, Bing-Kai1
2016-10-01
Source PublicationPLANT JOURNAL
Volume88Issue:1Pages:26-42
AbstractGlycosylation of monolignols has been found to be widespread in land plants since the 1970s. However, whether monolignol glycosylation is crucial for cell wall lignification and how it exerts effects are still unknown. Here, we report the identification of a mutant ugt72b1 showing aggravated and ectopic lignification in floral stems along with arrested growth and anthocyanin accumulation. Histochemical assays and thioacidolysis analysis confirmed the enhanced lignification and increased lignin biosynthesis in the ugt72b1 mutant. The loss of UDP-glycosyltransferase UGT72B1 function was responsible for the lignification phenotype, as demonstrated by complementation experiments. Enzyme activity analysis indicated that UGT72B1 could catalyze the glucose conjugation of monolignols, especially coniferyl alcohol and coniferyl aldehyde, which was confirmed by analyzing monolignol glucosides of UGT72B1 transgenic plants. Furthermore, the UGT72B1 gene was strongly expressed in young stem tissues, especially xylem tissues. However, UGT72B1 paralogs, such as UGT72B2 and UGT72B3, had weak enzyme activity toward monolignols and weak expression in stem tissues. Transcriptomic profiling showed that UGT72B1 knockout resulted in extensively increased transcript levels of genes involved in monolignol biosynthesis, lignin polymerization and cell wall-related transcription factors, which was confirmed by quantitative real-time PCR assays. These results provided evidence that monolignol glucosylation catalyzed by UGT72B1 was essential for normal cell wall lignification, thus offering insight into the molecular mechanism of cell wall development and cell wall lignification.
SubtypeArticle
KeywordArabidopsis Thaliana Monolignols Glycosylation Glycosyltransferase Cell Wall Lignification Transcriptome
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1111/tpj.13229
WOS KeywordNAC TRANSCRIPTION FACTORS ; LIGNIN BIOSYNTHESIS ; BETA-GLUCOSIDASES ; CONIFERIN ; TISSUE ; GLUCOSYLTRANSFERASE ; METABOLISM ; TRANSPORT ; PLANTS ; GROWTH
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
Funding OrganizationNational Natural Science Foundation of China(31570299 ; 91217301)
WOS SubjectPlant Sciences
WOS IDWOS:000388442100003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/9104
Collection作物分子育种团队
Affiliation1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ China, Jinan 250100, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Lin, Ji-Shan,Huang, Xu-Xu,Li, Qin,et al. UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana[J]. PLANT JOURNAL,2016,88(1):26-42.
APA Lin, Ji-Shan.,Huang, Xu-Xu.,Li, Qin.,Cao, Yingping.,Bao, Yan.,...&Hou, Bing-Kai.(2016).UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana.PLANT JOURNAL,88(1),26-42.
MLA Lin, Ji-Shan,et al."UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana".PLANT JOURNAL 88.1(2016):26-42.
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