UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana

doi:10.1111/tpj.13229

QIBEBT-IR > 能源作物分子育种研究组

	UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana
	Lin, Ji-Shan 1; Huang, Xu-Xu 1; Li, Qin 1; Cao, Yingping2 ; Bao, Yan2 ; Meng, Xia-Fei 1; Li, Yan-Jie 1; Fu, Chunxiang2 ; Hou, Bing-Kai 1
	2016-10-01
发表期刊	PLANT JOURNAL
卷号	88 期号:1 页码:26-42
摘要	Glycosylation of monolignols has been found to be widespread in land plants since the 1970s. However, whether monolignol glycosylation is crucial for cell wall lignification and how it exerts effects are still unknown. Here, we report the identification of a mutant ugt72b1 showing aggravated and ectopic lignification in floral stems along with arrested growth and anthocyanin accumulation. Histochemical assays and thioacidolysis analysis confirmed the enhanced lignification and increased lignin biosynthesis in the ugt72b1 mutant. The loss of UDP-glycosyltransferase UGT72B1 function was responsible for the lignification phenotype, as demonstrated by complementation experiments. Enzyme activity analysis indicated that UGT72B1 could catalyze the glucose conjugation of monolignols, especially coniferyl alcohol and coniferyl aldehyde, which was confirmed by analyzing monolignol glucosides of UGT72B1 transgenic plants. Furthermore, the UGT72B1 gene was strongly expressed in young stem tissues, especially xylem tissues. However, UGT72B1 paralogs, such as UGT72B2 and UGT72B3, had weak enzyme activity toward monolignols and weak expression in stem tissues. Transcriptomic profiling showed that UGT72B1 knockout resulted in extensively increased transcript levels of genes involved in monolignol biosynthesis, lignin polymerization and cell wall-related transcription factors, which was confirmed by quantitative real-time PCR assays. These results provided evidence that monolignol glucosylation catalyzed by UGT72B1 was essential for normal cell wall lignification, thus offering insight into the molecular mechanism of cell wall development and cell wall lignification.
文章类型	Article
关键词	Arabidopsis Thaliana Monolignols Glycosylation Glycosyltransferase Cell Wall Lignification Transcriptome
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1111/tpj.13229
关键词[WOS]	NAC TRANSCRIPTION FACTORS ; LIGNIN BIOSYNTHESIS ; BETA-GLUCOSIDASES ; CONIFERIN ; TISSUE ; GLUCOSYLTRANSFERASE ; METABOLISM ; TRANSPORT ; PLANTS ; GROWTH
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
项目资助者	National Natural Science Foundation of China(31570299 ; 91217301)
WOS类目	Plant Sciences
WOS记录号	WOS:000388442100003
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9104
专题	能源作物分子育种研究组
作者单位	1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ China, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Lin, Ji-Shan,Huang, Xu-Xu,Li, Qin,et al. UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana[J]. PLANT JOURNAL,2016,88(1):26-42.
APA	Lin, Ji-Shan.,Huang, Xu-Xu.,Li, Qin.,Cao, Yingping.,Bao, Yan.,...&Hou, Bing-Kai.(2016).UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana.PLANT JOURNAL,88(1),26-42.
MLA	Lin, Ji-Shan,et al."UDP-glycosyltransferase 72B1 catalyzes the glucose conjugation of monolignols and is essential for the normal cell wall lignification in Arabidopsis thaliana".PLANT JOURNAL 88.1(2016):26-42.