Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism

doi:10.1007/s00253-016-7719-x

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	Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism
	Chen, Quan 1; Toh, Phyllicia 2; Sun, Yue 1; Latiff, Sarah Maria Abdul 2; Hoi, Aina 2; Xian, Mo 1; Zhang, Haibo 1; Nian, Rui 1; Zhang, Wei 2; Gagnon, Pete 2
	2016-12-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	100 期号:23 页码:9933-9941
摘要	Octanoic acid (OA) precipitation has long been used in protein purification. Recently, we reported a new cell culture clarification method for immunoglobulin G (IgG) purification, employing an advance elimination of chromatin heteroaggregates with a hybrid OA-solid phase system. This treatment reduced DNA more than 3 logs, histone below the detection limit (LOD), and non-histone host cell proteins (nh-HCP) by 90 % while conserving more than 90 % of the IgG monomer. In this study, we further investigated the conservation of IgG monomer and antibody light chain (LC) to the addition of OA/OA-solid phase complex, with or without histone and DNA in different combinations. The results showed that highly basic histone protein was the prime target in OA/OA-solid phase precipitation system for IgG purification, and the selective conservation of IgG monomer in this system was histone dependent. Our findings partially support the idea that OA works by sticking to electropositive hydrophobic domains on proteins, reducing their solubility, and causing them to agglomerate into large particles that precipitate from solution. Our findings also provide a new perspective for IgG purification and emphasize the necessity to re-examine the roles of various host contaminants in IgG purification.
文章类型	Article
关键词	Octanoic Acid Agglomeration Histone Igg Light Chain
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-016-7719-x
关键词[WOS]	CHO-CELL LINES ; MONOCLONAL-ANTIBODY ; CAPRYLIC-ACID ; IMMUNOGLOBULIN-G ; LIGHT-CHAIN ; PROTEIN-A ; PURIFICATION ; CHROMATIN ; CHROMATOGRAPHY ; PERFORMANCE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	QIBEBT (Qingdao Institute of Bioenergy and Bioprocess Technology) Start-up Fund(Y571061905) ; Biomedical Research Council of A*STAR ; Exploit Technologies Pte Ltd., of Singapore(ETPL/12-R15GAP-0009)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000387656500011
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9097
专题	生物基材料组群
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.ASTAR, Bioproc Technol Inst, Singapore, Singapore
推荐引用方式 GB/T 7714	Chen, Quan,Toh, Phyllicia,Sun, Yue,et al. Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(23):9933-9941.
APA	Chen, Quan.,Toh, Phyllicia.,Sun, Yue.,Latiff, Sarah Maria Abdul.,Hoi, Aina.,...&Gagnon, Pete.(2016).Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(23),9933-9941.
MLA	Chen, Quan,et al."Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.23(2016):9933-9941.