A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

doi:10.1016/j.carbpol.2016.06.059

QIBEBT-IR > 分子微生物工程研究组

	A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast
	Wang, Da 1,3; Li, Fu-Li1,2 ; Wang, Shi-An1
	2016-10-20
发表期刊	CARBOHYDRATE POLYMERS
卷号	151 页码:1220-1226
摘要	Commercial fructo-oligosaccharides (FOS) are predominantly produced from sucrose by transfructosylation process that presents a maximum theoretical yield below 0.60 g(Fos) g(Sucrose)(-1). To obtain high-content FOS, costly purification is generally employed. Additionally, high-content FOS can be produced from inulin by using endo-inulinases. However, commercial endo-inulinases have not been extensively used in scale-up production of FOS. In the present study, a one-step bioprocess that integrated endo-inulinase production, FOS fermentation, and non-FOS sugars removal into one reactor was proposed to produce high-content FOS from inulin. The bioprocess was implemented by a recombinant yeast strain JZH Delta S-TSC, in which a heterologous endo-inulinase gene was expressed and the inherent invertase gene SUC2 was disrupted. FOS fermentation at 40 degrees C from 200 g/L chicory inulin presented the maximun titer, yield, and productivity of 180.2 +/- 0.8 g/L, 0.9 g(Fos) g(Inulin)(-1), and 7.51 +/- 0.03 g/L/h, respectively. This study demonstrated that the one-step bioprocess was simple and highly efficient. (C) 2016 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Fructo-oligosaccharides Inulin One-step Bioprocess Yeast
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.carbpol.2016.06.059
关键词[WOS]	INULOOLIGOSACCHARIDE PRODUCTION ; ASPERGILLUS-FICUUM ; ENDOINULINASE ; FRUCTOOLIGOSACCHARIDES ; PURIFICATION ; GENE ; EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Polymer Science
项目资助者	Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; Science and Technology Commission Foundation of Qingdao(13-1-4-193-jch)
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000380943900137
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8433
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Da,Li, Fu-Li,Wang, Shi-An. A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast[J]. CARBOHYDRATE POLYMERS,2016,151:1220-1226.
APA	Wang, Da,Li, Fu-Li,&Wang, Shi-An.(2016).A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast.CARBOHYDRATE POLYMERS,151,1220-1226.
MLA	Wang, Da,et al."A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast".CARBOHYDRATE POLYMERS 151(2016):1220-1226.