| Stable isotope labeling to study the nitrogen metabolism in microcystin biosynthesis | |
| Han, Zhen-Lian1,2,3,4; Shi, Xiao-Shuang4; Ji, Yue-Tong4; Tan, Xiao-Ming4; Bai, Fa-Li4; Yuan, Xian-Zheng1,4; Wang, Yi-Qian3; Guo, Rong-Bo4 | |
| 2016 | |
| 发表期刊 | RSC ADVANCES
 |
| 卷号 | 6期号:52页码:46806-46812 |
| 摘要 | Cyanobacterial blooms have occurred frequently in the surface waters of the world, posing a potential threat to humans and ecosystems due to their toxic secondary metabolite, microcystin. Genetic tools cannot easily explain the biosynthesis of MC in specific strains due to the iterative nature of MC production and environmental factors. In this study, the fully N-15-labeled MC-LR was biosynthesized successfully in Microcystis aeruginosa by in vivo stable isotopic enrichment. The biosynthesis and metabolic flux of MC-LR were explored through Raman spectroscopy and liquid chromatography-mass spectrometry (LC-MS). The results showed that the essential amino acids that were incorporated into MC-LR were all labeled by N-15. The Raman spectra revealed that most of the bands in 6th cell transfer MC-LR were shifted to lower wavenumbers due to the substitution by the heavier isotope N-15. MS indicated that fully N-15-labeled Arg and MC-LR were the dominant species from the 2nd and 6th cell transfer M. aeruginosa, respectively. Through MS-MS fragmentation analysis, Adda was the first unit to be synthesized, followed by Ala, MeAsp, Arg, Leu, Mdha and Glu. These results provide new evidence and an ideal tool to explore the biosynthesis of MC-LR in the specific strain of M. aeruginosa. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1039/c6ra03031a |
| 关键词[WOS] | REAL-TIME PCR ; TOXIC MICROCYSTIS ; RAMAN-SPECTROSCOPY ; AERUGINOSA ; LR ; QUANTIFICATION ; LAKE ; DIFFERENTIATION ; IDENTIFICATION ; QUANTITATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| 项目资助者 | National Natural Science Foundation of China(51108443 ; Applied Basic Research Foundation of Qingdao City(14-2-4-45-jch ; Taishan Scholar Program of Shandong Province and Youth Innovation Promotion Association, CAS ; 51478453) ; 14-2-4-21-jch) |
| WOS类目 | Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000377254800083 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8234 |
| 专题 | 工业生物燃气研究组 |
| 作者单位 | 1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266100, Peoples R China 2.Qingdao Univ, Coll Mat Sci & Engn, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Univ, Cultivat Base State Key Lab, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Zhen-Lian,Shi, Xiao-Shuang,Ji, Yue-Tong,et al. Stable isotope labeling to study the nitrogen metabolism in microcystin biosynthesis[J]. RSC ADVANCES,2016,6(52):46806-46812. |
| APA | Han, Zhen-Lian.,Shi, Xiao-Shuang.,Ji, Yue-Tong.,Tan, Xiao-Ming.,Bai, Fa-Li.,...&Guo, Rong-Bo.(2016).Stable isotope labeling to study the nitrogen metabolism in microcystin biosynthesis.RSC ADVANCES,6(52),46806-46812. |
| MLA | Han, Zhen-Lian,et al."Stable isotope labeling to study the nitrogen metabolism in microcystin biosynthesis".RSC ADVANCES 6.52(2016):46806-46812. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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