A bladder cancer microenvironment simulation system based on a microfluidic co-culture model

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	A bladder cancer microenvironment simulation system based on a microfluidic co-culture model
	Liu, Peng-fei 1,2; Cao, Yan-wei 1,3; Zhang, Shu-dong 4; Zhao, Yang 1,2; Liu, Xiao-guang 1,2; Shi, Hao-qing 1,2; Hu, Ke-yao 1,2; Zhu, Guan-qun 1,2; Ma, Bo5,6 ; Niu, Hai-tao 1,3
	2015-11-10
发表期刊	ONCOTARGET
卷号	6 期号:35 页码:37695-37705
摘要	A tumor microenvironment may promote tumor metastasis and progression through the dynamic interplay between neoplastic cells and stromal cells. In this work, the most representative and significant stromal cells, fibroblasts, endothelial cells, and macrophages were used as vital component elements and combined with bladder cancer cells to construct a bladder cancer microenvironment simulation system. This is the first report to explore bladder cancer microenvironments based on 4 types of cells co-cultured simultaneously. This simulation system comprises perfusion equipment, matrigel channel units, a medium channel and four indirect contact culture chambers, allowing four types of cells to simultaneously interact through soluble biological factors and metabolites. With this system, bladder cancer cells (T24) with a tendency to form a 'reticular' structure under 3 dimensional culture conditions were observed in real time. The microenvironment characteristics of paracrine interactions and cell motility were successfully simulated in this system. The phenotype change process in stromal cells was successfully reproduced in this system by testing the macrophage effector molecule Arg-1. Arg-1 was highly expressed in the simulated tumor microenvironment group. To develop "precision medicine" in bladder cancer therapy, bladder cancer cells were treated with different clinical 'neo-adjuvant' chemotherapy schemes in this system, and their sensitivity differences were fully reflected. This work provides a preliminary foundation for neo-adjuvant chemotherapy in bladder cancer, a theoretical foundation for tumor microenvironment simulation and promotes individual therapy in bladder cancer patients.
文章类型	Article
关键词	Microfluidic Device Bladder Cancer Microenvironment Three-dimensional Culture Co-culture Drug Sensitivity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	TUMOR MICROENVIRONMENT ; BREAST-CANCER ; ANTICANCER THERAPIES ; VASCULOGENIC MIMICRY ; ANGIOGENIC SWITCH ; DRUG-RESISTANCE ; LUNG-CANCER ; CELLS ; HETEROGENEITY ; EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Oncology ; Cell Biology
项目资助者	National Natural Science Foundation of China(30901481 ; Wu JiePing Medical Foundation(320.6750.13261) ; Natural Science Foundation of Shandong Province, China(ZR2014HM088) ; 81372752 ; 81472411)
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000366113200055
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6837
专题	单细胞中心组群
作者单位	1.Qingdao Univ, Affiliated Hosp, Dept Urol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Univ, Coll Med, Qingdao 266071, Shandong, Peoples R China 3.Key Urol Lab Qingdao City, Qingdao, Shandong, Peoples R China 4.Peking Univ, Hosp 3, Dept Urol, Beijing 100871, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China 6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Peng-fei,Cao, Yan-wei,Zhang, Shu-dong,et al. A bladder cancer microenvironment simulation system based on a microfluidic co-culture model[J]. ONCOTARGET,2015,6(35):37695-37705.
APA	Liu, Peng-fei.,Cao, Yan-wei.,Zhang, Shu-dong.,Zhao, Yang.,Liu, Xiao-guang.,...&Niu, Hai-tao.(2015).A bladder cancer microenvironment simulation system based on a microfluidic co-culture model.ONCOTARGET,6(35),37695-37705.
MLA	Liu, Peng-fei,et al."A bladder cancer microenvironment simulation system based on a microfluidic co-culture model".ONCOTARGET 6.35(2015):37695-37705.