Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli

doi:10.1007/s00726-014-1892-y

QIBEBT-IR > 分子微生物工程研究组

	Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli
	Chang, Ziwei 1,2; Lu, Ming 3; Ma, Yunqi 1,2; Kwag, Dong-Geon 1,2; Kim, Seo-Hyun 1,2; Park, Ji-Min 1,2; Nam, Bo-Hye 4; Kim, Young-Ok 4; An, Cheul-Min 4; Li, Huayue 5; Jung, Jee H.5; Park, Jang-Su 1,2
	2015-03-01
发表期刊	AMINO ACIDS
卷号	47 期号:3 页码:579-587
摘要	Recombinant expression in Escherichia coli allows the simple, economical, and effective production of bioactive peptides. On the other hand, the production of native peptides, particularly those rich in disulfide bonds, is a major problem. Previous studies have reported that the use of carrier proteins for fusion expression can result in good peptide yields, but few are folded correctly. In this study, two transmembrane small proteins in E. coli, YoaJ and YkgR, which both orientate with their N-termini in cytoplasm and their C-termini in periplasm, were used for fusion expression. The recombinant production of two peptides, asteropsin A (ASPA) and beta-defensin (BD), was induced in the periplasm of E. coli using a selected carrier protein. Both peptides were expressed at high levels, at yields of approximately 5-10 mg/L of culture. Mass spectrometry showed that the resulting peptide had the same molecular weight as their natural forms. After purification, single peaks were observed by reversed phase high-performance liquid chromatography (RP-HPLC), demonstrating the absence of isoforms. Furthermore, cytoplasmically expressed fusion proteins with a carrier at their C-termini did not contain disulfide bonds. This study provides new carrier proteins for fusion expression of disulfide bond-rich peptides in E. coli.
文章类型	Article
关键词	Disulfide Bond Escherichia Coli Carrier Protein Mass Spectrometry
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00726-014-1892-y
关键词[WOS]	ANTIMICROBIAL PEPTIDES ; BACTERIAL PERIPLASM ; SYSTEM ; THIOREDOXIN ; ANTIBODIES ; ISOMERASE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000349390700013
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6726
专题	分子微生物工程研究组
作者单位	1.Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea 2.Pusan Natl Univ, Chem Inst Funct Mat, Pusan 609735, South Korea 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Natl Fisheries Res & Dev Inst, Aquaculture Ind Dept, Biotechnol Res Div, Pusan 619902, South Korea 5.Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea
推荐引用方式 GB/T 7714	Chang, Ziwei,Lu, Ming,Ma, Yunqi,et al. Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli[J]. AMINO ACIDS,2015,47(3):579-587.
APA	Chang, Ziwei.,Lu, Ming.,Ma, Yunqi.,Kwag, Dong-Geon.,Kim, Seo-Hyun.,...&Park, Jang-Su.(2015).Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli.AMINO ACIDS,47(3),579-587.
MLA	Chang, Ziwei,et al."Production of disulfide bond-rich peptides by fusion expression using small transmembrane proteins of Escherichia coli".AMINO ACIDS 47.3(2015):579-587.