Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

doi:10.1104/pp.113.214320

QIBEBT-IR > 单细胞中心组群

	Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery
	Dong, Hong-Po 1,2; Williams, Ernest 2; Wang, Da-zhi 3; Xie, Zhang-Xian 3; Hsia, Ru-ching 4; Jenck, Alizee 5; Halden, Rolf 5; Li, Jing6 ; Chen, Feng 2; Place, Allen R.2
	2013-06-01
发表期刊	PLANT PHYSIOLOGY
卷号	162 期号:2 页码:1110-1126
摘要	The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1104/pp.113.214320
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; BIOFUEL PRODUCTION ; GROWTH ; METABOLISM ; MICROALGAE ; SEAWATER ; ALGAE ; PURIFICATION ; DEPRIVATION
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000319819900044
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6699
专题	单细胞中心组群
作者单位	1.Jinan Univ, Res Ctr Harmful Algae & Marine Biol, Guangzhou 510632, Guangdong, Peoples R China 2.Univ Maryland, Inst Marine & Environm Technol, Ctr Environm Sci, Baltimore, MD 21202 USA 3.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China 4.Univ Maryland, Sch Dent, Dept Microbial Pathogenesis, Baltimore, MD 21202 USA 5.Arizona State Univ, Ctr Environm Secur, Biodesign Inst, Secur & Def Syst Initiat, Tempe, AZ 85287 USA 6.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Genome Ctr, CAS Key Lab Biofuels,Shandong Key Lab Energy Gene, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Dong, Hong-Po,Williams, Ernest,Wang, Da-zhi,et al. Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery[J]. PLANT PHYSIOLOGY,2013,162(2):1110-1126.
APA	Dong, Hong-Po.,Williams, Ernest.,Wang, Da-zhi.,Xie, Zhang-Xian.,Hsia, Ru-ching.,...&Place, Allen R..(2013).Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery.PLANT PHYSIOLOGY,162(2),1110-1126.
MLA	Dong, Hong-Po,et al."Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery".PLANT PHYSIOLOGY 162.2(2013):1110-1126.