Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate

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	Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate
	Zhang, Ke-jun 1,2; Liu, Wu-yi 1
	2011-09-01
发表期刊	INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
卷号	6 期号:9 页码:4006-4015
摘要	The cyclic voltammetry and ultraviolet-visible absorption spectrometry have proved to be powerful electrochemical means for the research of interactions between G-quadruplex DNA (G4-DNA) and chemical anti-carcinogens. In this study, the electrochemical behavior of (R)-/(S)-2-(5-fluorouracil-1-acetyl) amido-1,5-dimethyl glutarate (i.e., (R)-5FUGlu and (S)-5FUGlu) and the interaction of (R)/(S)-2-(5-fluorouracil-1-acetyl) amido-1,5-dimethyl glutarate with DNA using Fe(CN)(6)(3-/4-) as electroactive indicator were investigated, and the compounds were assayed for anticancer activities in HL-60 cells. The experiment showed that the interaction between (R)-/(S)-2-(5-fluorouracil-1-acetyl) amido-1,5-dimethyl glutarate and DNA were electrostatic, and the binding equilibrium constant KG4-DNA of the compounds is closely related to the configuration. It was observed that the binding equilibrium constant KG4-DNA of (R)-5FUGlu was about 2 times larger than that of (S)-5FUGlu. The inhibition rates of the different concentration of (R)-5FUGlu in tumor cell line HL-60 cells were larger than those of (S)-5FUGlu, which gave a good agreement with the above-mentioned electrochemical results.
文章类型	Article
关键词	G-quadruplex Dna Gold Electrode (r)-5fuglu (s)-5fuglu Cyclic Voltammetry
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	TELOMERIC G-QUADRUPLEX ; SMALL-MOLECULE ; ANTITUMOR-ACTIVITY ; DRUG DESIGN ; BASE PAIRS ; 5-FLUOROURACIL ; COMPLEXES ; LIGANDS ; BINDING ; DERIVATIVES
收录类别	SCI
语种	英语
WOS研究方向	Electrochemistry
WOS类目	Electrochemistry
WOS记录号	WOS:000297501400024
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6637
专题	仿生与固态能源系统研究组
作者单位	1.Fuyang Normal Coll, Dept Biol Sci, Fuyang City 236041, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ke-jun,Liu, Wu-yi. Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate[J]. INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,2011,6(9):4006-4015.
APA	Zhang, Ke-jun,&Liu, Wu-yi.(2011).Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate.INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,6(9),4006-4015.
MLA	Zhang, Ke-jun,et al."Electrochemical and Spectroscopic Study on the Interaction Between G-Quadruplex DNA and (R)-/(S)-2-(5-Fluorouracil-1-Acetyl) Amido-1, 5-Dimethyl Glutarate".INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE 6.9(2011):4006-4015.