Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

doi:10.1038/srep14446

QIBEBT-IR > 代谢物组学研究组

	Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions
	Ma, Zengxin 1,3,4; Tan, Yanzhen 1,3; Cui, Guzhen 1,3; Feng, Yingang 1,3; Cui, Qiu 1,2,3; Song, Xiaojin 1,3
	2015-09-25
发表期刊	SCIENTIFIC REPORTS
卷号	5
摘要	Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid and DHA content. In this study, high-throughput RNA sequencing technology was employed to explore the acclimatization of this DHA producer under cold stress at the transcriptional level. The overall de novo assembly of the cDNA sequence data generated 29,783 unigenes, with an average length of 1,200 bp. In total, 13,245 unigenes were annotated in at least one database. A comparative genomic analysis between normal conditions and cold stress revealed that 2,013 genes were differentially expressed during the growth stage, while 2,071 genes were differentially expressed during the lipid accumulation stage. Further functional categorization and analyses showed some differentially expressed genes were involved in processes crucial to cold acclimation, such as signal transduction, cellular component biogenesis, and carbohydrate and lipid metabolism. A brief survey of the transcripts obtained in response to cold stress underlines the survival strategy of Aurantiochytrium; of these transcripts, many directly or indirectly influence the lipid composition. This is the first study to perform a transcriptomic analysis of the Aurantiochytrium under low temperature conditions. Our results will help to enhance DHA production by Aurantiochytrium in the future.
文章类型	Article
WOS标题词	Science & Technology
DOI	10.1038/srep14446
关键词[WOS]	FATTY-ACID BIOSYNTHESIS ; RNA-SEQ DATA ; DOCOSAHEXAENOIC ACID ; SACCHAROMYCES-CEREVISIAE ; SCHIZOCHYTRIUM-LIMACINUM ; METABOLIC-RESPONSE ; STRESS-ADAPTATION ; OXIDATIVE STRESS ; REFERENCE GENOME ; LIPID-CONTENT
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000361733700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6601
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 3.Qingdao Engn Lab Single Cell Oil, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ma, Zengxin,Tan, Yanzhen,Cui, Guzhen,et al. Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions[J]. SCIENTIFIC REPORTS,2015,5.
APA	Ma, Zengxin,Tan, Yanzhen,Cui, Guzhen,Feng, Yingang,Cui, Qiu,&Song, Xiaojin.(2015).Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions.SCIENTIFIC REPORTS,5.
MLA	Ma, Zengxin,et al."Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions".SCIENTIFIC REPORTS 5(2015).