Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum

doi:10.1007/s12104-015-9601-7

QIBEBT-IR > 代谢物组学研究组

	Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum
	Ding, Xiaoke 1,2; Chen, Chao2,3 ; Cui, Qiu2,3,4 ; Li, Wenli 1; Feng, Yingang2,3
	2015-10-01
发表期刊	BIOMOLECULAR NMR ASSIGNMENTS
卷号	9 期号:2 页码:321-324
摘要	The cellulosome of Clostridium thermocellum is an elegant and efficient multi-enzyme complex for degrading lignocellulose. The cellulosome contains several dozens of carbohydrate hydrolysis enzymes, which are regulated by the presence of environmental substrates through several pairs of sigma and anti-sigma factors. The anti-sigma factors sense the presence of substrates and transduce the signals into the cell. The sigma factors are then released from the corresponding anti-sigma factors, and they recruit RNA polymerase to transcribe specific cellulosomal genes. However, it is not clear how the extracellular signals are transduced into the cell by the anti-sigma factors. The anti-sigma factors of C. thermocellum contain an N-terminal intracellular domain, a trans-membrane helix, a periplasmic domain, a proline-rich region which is probably required for crossing the cell wall, and a C-terminal carbohydrate-binding domain or glycoside hydrolase domain. The periplasmic domain may play a key role in signal transduction; however, its three-dimensional structure is still unknown. Here we report the NMR resonance assignments of the periplasmic domain of anti-sigma factor RsgI2 from C. thermocellum as a basis for further structural determination and functional studies.
文章类型	Article
关键词	Anti-sigma Factor Clostridium Thermocellum Nmr Chemical Shift Assignment Periplasmic Domain
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1007/s12104-015-9601-7
收录类别	SCI
语种	英语
WOS研究方向	Biophysics ; Spectroscopy
WOS类目	Biophysics ; Spectroscopy
WOS记录号	WOS:000361440100022
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6600
专题	代谢物组学研究组
作者单位	1.Ocean Univ China, Minist Educ, Sch Med & Pharm, Key Lab Marine Drugs, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ding, Xiaoke,Chen, Chao,Cui, Qiu,et al. Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum[J]. BIOMOLECULAR NMR ASSIGNMENTS,2015,9(2):321-324.
APA	Ding, Xiaoke,Chen, Chao,Cui, Qiu,Li, Wenli,&Feng, Yingang.(2015).Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum.BIOMOLECULAR NMR ASSIGNMENTS,9(2),321-324.
MLA	Ding, Xiaoke,et al."Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum".BIOMOLECULAR NMR ASSIGNMENTS 9.2(2015):321-324.