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Isolation and characterization of a chromium-resistant bacterium Serratia sp Cr-10 from a chromate-contaminated site
Zhang, Kundi; Li, Fuli
2011-05-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume90Issue:3Pages:1163-1169
Abstract

A novel bacterium, Cr-10, was isolated from a chromium-contaminated site and capable of removing toxic chromium species from solution by reducing hexavalent chromium to an insoluble precipitate. Sequence analysis of 16S rRNA gene of strain Cr-10 showed that it was most closely related to Serratia rubidaea JCM 1240(T) (97.68%). Physiological and chemotaxonomic data also supported that strain Cr-10 was identified as Serratia sp., a genus which was never specially reported chromate-resistant before. Serratia sp., Cr-10 was tolerant to a concentration of 1,500 mg Cr(VI) L-1, which was the highest level reported until now. The optimum pH and temperature for reduction of Cr(VI) by Serratia sp. Cr-10 were found to be 7.0 and 37 A degrees C, respectively. The Cr(VI) reduction was significantly influenced by additional carbon sources, and among them fructose and lactose offered maximum reduction, with a rate of 0.28 and 0.25 mg Cr(VI) L-1 h(-1), respectively. The cell-free extracts and filtrate of the culture were able to reduce Cr(VI) while concentration of total chromium remained stable in the process, indicating that the enzyme-catalyzed mechanism was applied in Cr(VI) reduction by the isolate. Additionally, it was found that there was hardly any chromium on the cell surface of the strain, further supporting that reduction, rather than bioadsorption, plays a major role in the Cr(VI) removal.

SubtypeArticle
KeywordCr(Vi) Reduction Serratia Sp. Batch Reduction Bioremediation
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-011-3120-y
WOS KeywordCELL-FREE-EXTRACT ; PSEUDOMONAS-AERUGINOSA ; HEXAVALENT CHROMIUM ; ESCHERICHIA-COLI ; REDUCTION ; IDENTIFICATION ; GENES ; NOV
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000289520000036
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6573
Collection分子微生物工程研究组
AffiliationChinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Kundi,Li, Fuli. Isolation and characterization of a chromium-resistant bacterium Serratia sp Cr-10 from a chromate-contaminated site[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,90(3):1163-1169.
APA Zhang, Kundi,&Li, Fuli.(2011).Isolation and characterization of a chromium-resistant bacterium Serratia sp Cr-10 from a chromate-contaminated site.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,90(3),1163-1169.
MLA Zhang, Kundi,et al."Isolation and characterization of a chromium-resistant bacterium Serratia sp Cr-10 from a chromate-contaminated site".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 90.3(2011):1163-1169.
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