| De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation | |
| Duan, Yangkai; Zhu, Zhi; Cai, Ke; Tan, Xiaoming; Lu, Xuefeng | |
| 2011-05-23 | |
| 发表期刊 | PLOS ONE
 |
| 卷号 | 6期号:5页码:e20265 |
| 摘要 | Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are mainly obtained from oilseed plants and microalgae. A sustainable supply of TAGs is a major bottleneck for current biodiesel production. Here we report the de novo biosynthesis of FAEEs from glucose, which can be derived from lignocellulosic biomass, in genetically engineered Escherichia coli by introduction of the ethanol-producing pathway from Zymomonas mobilis, genetic manipulation to increase the pool of fatty acyl-CoA, and heterologous expression of acyl-coenzyme A: diacylglycerol acyltransferase from Acinetobacter baylyi. An optimized fed-batch microbial fermentation of the modified E. coli strain yielded a titer of 922 mg L(-1) FAEEs that consisted primarily of ethyl palmitate, -oleate, -myristate and -palmitoleate.; Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are mainly obtained from oilseed plants and microalgae. A sustainable supply of TAGs is a major bottleneck for current biodiesel production. Here we report the de novo biosynthesis of FAEEs from glucose, which can be derived from lignocellulosic biomass, in genetically engineered Escherichia coli by introduction of the ethanol-producing pathway from Zymomonas mobilis, genetic manipulation to increase the pool of fatty acyl-CoA, and heterologous expression of acyl-coenzyme A: diacylglycerol acyltransferase from Acinetobacter baylyi. An optimized fed-batch microbial fermentation of the modified E. coli strain yielded a titer of 922 mg L(-1) FAEEs that consisted primarily of ethyl palmitate, -oleate, -myristate and -palmitoleate. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0020265 |
| 关键词[WOS] | BIOMASS ; OVERPRODUCTION ; BIOFUELS ; ALKANES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000291005200044 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/656 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Yangkai,Zhu, Zhi,Cai, Ke,et al. De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation[J]. PLOS ONE,2011,6(5):e20265. |
| APA | Duan, Yangkai,Zhu, Zhi,Cai, Ke,Tan, Xiaoming,&Lu, Xuefeng.(2011).De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation.PLOS ONE,6(5),e20265. |
| MLA | Duan, Yangkai,et al."De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation".PLOS ONE 6.5(2011):e20265. |
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