| The utility of mass spectrometry-based proteomic data for validation of novel alternative splice forms reconstructed from RNA-Seq data: a preliminary assessment | |
| Ning, Kang1,3; Nesvizhskii, Alexey I.1,2 | |
| 2010-12-14 | |
| 发表期刊 | BMC BIOINFORMATICS
 |
| 卷号 | 11期号:1页码:1 |
| 摘要 | Background: Most mass spectrometry (MS) based proteomic studies depend on searching acquired tandem mass (MS/MS) spectra against databases of known protein sequences. In these experiments, however, a large number of high quality spectra remain unassigned. These spectra may correspond to novel peptides not present in the database, especially those corresponding to novel alternative splice (AS) forms. Recently, fast and comprehensive profiling of mammalian genomes using deep sequencing (i.e. RNA-Seq) has become possible. MS-based proteomics can potentially be used as an aid for protein-level validation of novel AS events observed in RNA-Seq data. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1186/1471-2105-11-S11-S14 |
| 关键词[WOS] | PEPTIDE IDENTIFICATION ; TANDEM ; GENOME ; GENE ; PROTEINS ; SPECTRA ; EVENTS ; INDEX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology |
| WOS记录号 | WOS:000290219200014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6534 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA 2.Univ Michigan, Ctr Computat Biol & Med, Ann Arbor, MI 48109 USA 3.Chinese Acad Sci, BioEnergy Genome Ctr, Qingdao Inst BioEnergy & Bioproc Technol, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, Kang,Nesvizhskii, Alexey I.. The utility of mass spectrometry-based proteomic data for validation of novel alternative splice forms reconstructed from RNA-Seq data: a preliminary assessment[J]. BMC BIOINFORMATICS,2010,11(1):1. |
| APA | Ning, Kang,&Nesvizhskii, Alexey I..(2010).The utility of mass spectrometry-based proteomic data for validation of novel alternative splice forms reconstructed from RNA-Seq data: a preliminary assessment.BMC BIOINFORMATICS,11(1),1. |
| MLA | Ning, Kang,et al."The utility of mass spectrometry-based proteomic data for validation of novel alternative splice forms reconstructed from RNA-Seq data: a preliminary assessment".BMC BIOINFORMATICS 11.1(2010):1. |
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| The utility of mass （342KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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