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Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes
Xia, Fan1,2; Zuo, Xiaolei2; Yang, Renqiang3; Xiao, Yi2; Kang, Di2; Vallee-Belisle, Alexis2; Gong, Xiong1; Yuen, Jonathan D.1; Hsu, Ben B. Y.1; Heeger, Alan J.1; Plaxco, Kevin W.2
2010-06-15
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷号107期号:24页码:10837-10841
摘要We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise "folded" DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead.
文章类型Article
关键词Biosensor Aptamer Visual Detection Thrombin Detection Cocaine Detection
WOS标题词Science & Technology
DOI10.1073/pnas.1005632107
关键词[WOS]SEQUENCE-SPECIFIC DETECTION ; OPTICAL-PROPERTIES ; NUCLEIC-ACIDS ; STRANDED-DNA ; SENSOR ; APTAMERS ; COCAINE ; OLIGONUCLEOTIDES ; BIOSENSOR ; AFFINITY
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000278807400014
引用统计
被引频次:356[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6530
专题先进有机功能材料团队
作者单位1.Univ Calif Santa Barbara, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA
2.Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
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Xia, Fan,Zuo, Xiaolei,Yang, Renqiang,et al. Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(24):10837-10841.
APA Xia, Fan.,Zuo, Xiaolei.,Yang, Renqiang.,Xiao, Yi.,Kang, Di.,...&Plaxco, Kevin W..(2010).Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(24),10837-10841.
MLA Xia, Fan,et al."Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.24(2010):10837-10841.
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