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Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes
Xia, Fan1,2; Zuo, Xiaolei2; Yang, Renqiang3; Xiao, Yi2; Kang, Di2; Vallee-Belisle, Alexis2; Gong, Xiong1; Yuen, Jonathan D.1; Hsu, Ben B. Y.1; Heeger, Alan J.1; Plaxco, Kevin W.2
2010-06-15
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume107Issue:24Pages:10837-10841
AbstractWe have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise "folded" DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead.
SubtypeArticle
KeywordBiosensor Aptamer Visual Detection Thrombin Detection Cocaine Detection
WOS HeadingsScience & Technology
DOI10.1073/pnas.1005632107
WOS KeywordSEQUENCE-SPECIFIC DETECTION ; OPTICAL-PROPERTIES ; NUCLEIC-ACIDS ; STRANDED-DNA ; SENSOR ; APTAMERS ; COCAINE ; OLIGONUCLEOTIDES ; BIOSENSOR ; AFFINITY
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000278807400014
Citation statistics
Cited Times:394[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6530
Collection先进有机功能材料团队
Affiliation1.Univ Calif Santa Barbara, Ctr Polymers & Organ Solids, Santa Barbara, CA 93106 USA
2.Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Xia, Fan,Zuo, Xiaolei,Yang, Renqiang,et al. Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(24):10837-10841.
APA Xia, Fan.,Zuo, Xiaolei.,Yang, Renqiang.,Xiao, Yi.,Kang, Di.,...&Plaxco, Kevin W..(2010).Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(24),10837-10841.
MLA Xia, Fan,et al."Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.24(2010):10837-10841.
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