Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores

doi:10.3389/fmicb.2015.00950

QIBEBT-IR > 分子微生物工程研究组

	Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores
	Liu, Zhen 1,2; Qiao, Kai 3; Tian, Lei 1,2; Zhang, Quan 3; Liu, Zi-Yong 1,2; Li, Fu-Li 1,2,3
	2015-09-09
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	6
摘要	Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase. To unravel the role of autolysis, an autolysin-coding gene, CA_C0554, was disrupted by using ClosTron system to obtain the mutant C. acetobutylicum lyc::int(72). The lower final cell density and faster cell density decrease rate of C. acetobutylicum ATCC 824 than those of C. acetobutylicum lyc::int(72) indicates that CA_C0554 was an important but not the sole autolysin-coding gene responding for the large-scale autolysis. Similar glucose utilization and solvents production but obvious lower cell density of C. acetobutylicum ATCC 824 comparing to C. acetobutylicum lyc::int(72) suggests that lysed C. acetobutylicum ATCC 824 cells were metabolic inactive. On the contrary, the spore density of C. acetobutylicum ATCC 824 is 26.1% higher than that of C. acetobutylicum lyc::int(72) in the final culture broth of batch fermentation. We speculated that spontaneous autolysis of metabolic-inactive cells provided nutrients for the sporulating cells. The present study suggests that one important biological role of spontaneous large-scale autolysis in C. acetobutylicum ATCC 824 batch fermentation is contributing to generation of more spores during sporulation.
文章类型	Article
关键词	Autolysis Sporulation Clostridium Acetobutylicum Clostron Autolysin
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fmicb.2015.00950
关键词[WOS]	BACILLUS-SUBTILIS 168 ; ESCHERICHIA-COLI ; DAIRY PROPIONIBACTERIA ; SOLVENT PRODUCTION ; LYTIC ENZYMES ; GENE ; PEPTIDOGLYCAN ; FERMENTATION ; PURIFICATION ; SPORULATION
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000361157900002
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6493
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Sinopec Fushun Res Inst Petr & Petrochem, Fushun, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhen,Qiao, Kai,Tian, Lei,et al. Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores[J]. FRONTIERS IN MICROBIOLOGY,2015,6.
APA	Liu, Zhen,Qiao, Kai,Tian, Lei,Zhang, Quan,Liu, Zi-Yong,&Li, Fu-Li.(2015).Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores.FRONTIERS IN MICROBIOLOGY,6.
MLA	Liu, Zhen,et al."Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores".FRONTIERS IN MICROBIOLOGY 6(2015).