Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain

doi:10.1039/c4mb00600c

QIBEBT-IR > 分子微生物工程研究组

	Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain
	Tan, Yang 1,2; Liu, Zi-Yong 1; Liu, Zhen1 ; Zheng, Hua-Jun 3; Li, Fu-Li1
	2015
发表期刊	MOLECULAR BIOSYSTEMS
卷号	11 期号:5 页码:1434-1442
摘要	The genome of Clostridium acetobutylicum contains the gene encoding CsrA, a carbon storage regulator. We investigated the function of CsrA in C. acetobutylicum by insertionally inactivating the encoding gene, CA_C2209 using the ClosTron. Disruption of csrA obviously decreases the growth of the organism and reduces the yield of acetone, butanol and ethanol (ABEs). Like the csrA in Escherichia coli, RNA-seq and beta-galactosidase analysis revealed that csrA in C. acetobutylicum was closely involved in regulating multiple pathways including flagella assembly, oligopeptide transporting, iron uptake, and central carbon metabolism. It has also been newly demonstrated that csrA in C. acetobutylicum is related to the regulation of pathways involved in the phosphotransferase transporting systems, synthesis of riboflavin, and stage III sporulation. This research represented the first investigation of global regulation by CsrA in the strain belonging to Gram-positive bacteria through transcriptome analysis and provided the important theoretical evidence for improving solvent production by transcriptor engineering in C. acetobutylicum.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1039/c4mb00600c
关键词[WOS]	RNA-BINDING PROTEIN ; ENTERICA SEROVAR TYPHIMURIUM ; CAROTOVORA SUBSP CAROTOVORA ; PSEUDOMONAS-AERUGINOSA RSMA ; AIRWAY EPITHELIAL-CELLS ; GLOBAL REGULATORY GENE ; ESCHERICHIA-COLI ; MESSENGER-RNA ; ATCC 824 ; BUTANOL PRODUCTION
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000353221100022
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6452
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioprocess Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Hlth & Dis Gen, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tan, Yang,Liu, Zi-Yong,Liu, Zhen,et al. Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain[J]. MOLECULAR BIOSYSTEMS,2015,11(5):1434-1442.
APA	Tan, Yang,Liu, Zi-Yong,Liu, Zhen,Zheng, Hua-Jun,&Li, Fu-Li.(2015).Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain.MOLECULAR BIOSYSTEMS,11(5),1434-1442.
MLA	Tan, Yang,et al."Comparative transcriptome analysis between csrA-disruption Clostridium acetobutylicum and its parent strain".MOLECULAR BIOSYSTEMS 11.5(2015):1434-1442.