Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair

doi:10.1016/j.mrfmmm.2014.07.010

QIBEBT-IR > 酶工程研究组

	Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair
	Li, Chongjie 1; Ma, Li 2,3; Mou, Shanli 4; Wang, Yibin 1; Zheng, Zhou 1; Liu, Fangming 1; Qi, Xiaoqing 1; An, Meiling 1; Chen, Hao 1; Miao, Jinlai 1,5
	2015-03-01
发表期刊	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
卷号	773 页码:37-42
摘要	Bacteria living in the Antarctic region have developed several adaptive features for growth and survival under extreme conditions. Chlamydomonas sp. ICE-Lis well adapted to high levels of solar UV radiation. A putative photolyase was identified in the Chlamydomonas sp. ICE-L transcriptome. The complete cDNA sequence was obtained by RACE-PCR. This PHR encoding includes a polypeptide of 579 amino acids with clear photolyase signatures belonging to class II CPD-photolyases, sharing a high degree of homology with Chlamydomonas reinhardtii (68%). Real-time PCR was performed to investigate the potential DNA damage and responses following UVB exposure. CPD photolyase mRNA expression level increased over 50-fold in response to UVB radiation for 6 h. Using photolyase complementation assay, we demonstrated that DNA photolyase increased photo-repair more than 116-fold in Escherichia coil strain SY2 under 100 mu w/cm(2) UVB radiation. To determine whether photolyase is active in vitro, CPD photolyase was over-expressed. It was shown that pyrimidine dimers were split by the action of PHR2. This study reports the unique structure and high activity of the enzyme. These findings are relevant for further understanding of molecular mechanisms of photo-reactivation, and will accelerate the utilization of photolyase in the medical field. (C) 2015 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Chlamydomonas Sp Ice-l Uv Photolyase Photo-reactivation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.mrfmmm.2014.07.010
关键词[WOS]	SP ICE-L ; HUMAN SKIN ; EXPRESSION ; APOPTOSIS ; STRESS ; PHOTOPROTECTION ; GENE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
WOS记录号	WOS:000351979200006
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6415
专题	酶工程研究组
作者单位	1.State Ocean Adm, Key Lab Marine Bioact Subst, Inst Oceanog 1, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China 5.State Key Lab Biol Fermentat Engn Beer Preparat, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Chongjie,Ma, Li,Mou, Shanli,et al. Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2015,773:37-42.
APA	Li, Chongjie.,Ma, Li.,Mou, Shanli.,Wang, Yibin.,Zheng, Zhou.,...&Miao, Jinlai.(2015).Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,773,37-42.
MLA	Li, Chongjie,et al."Cyclobutane pyrimidine dimers photolyase from extremophilic microalga: Remarkable UVB resistance and efficient DNA damage repair".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 773(2015):37-42.