Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus

doi:10.1007/s10126-011-9408-1

QIBEBT-IR > 资源植物与环境工程研究组

	Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus
	Ma, Yu-Bin1,2 ; Zhang, Zhi-Feng 1; Shao, Ming-Yu 1; Kang, Kyoung-Ho 3; Shi, Xiao-Li 1; Dong, Ying-Ping 1; Li, Jin-Long 1
	2012-04-01
发表期刊	MARINE BIOTECHNOLOGY
卷号	14 期号:2 页码:245-251
摘要	Sulfide is a natural, widely distributed, poisonous substance, and sulfide: quinone oxidoreductase (SQR) is responsible for the initial oxidation of sulfide in mitochondria. In this study, we examined the response of SQR to sulfide exposure (25, 50, and 150 mu M) at mRNA, protein, and enzyme activity levels in the body wall and hindgut of the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The results revealed SQR mRNA expression during sulfide exposure in the body wall and hindgut increased in a time- and concentration-dependent manner that increased significantly at 12 h and continuously increased with time. At the protein level, SQR expression in the two tissues showed a time-dependent relationship that increased significantly at 12 h in 50 mu M sulfide and 6 h in 150 mu M, and then continued to increase with time while no significant increase appeared after 25 mu M sulfide exposure. SQR enzyme activity in both tissues increased significantly in a time-dependent manner after 50 mu M sulfide exposure. We concluded that SQR expression could be induced by sulfide exposure and that the two tissues studied have dissimilar sulfide metabolic patterns. A U. unicinctus sulfide-induced detoxification mechanism was also discussed.
文章类型	Article
关键词	Mitochondria Sulfide Detoxification Sulfide: Quinone Oxidoreductase (Sqr) Urechis Unicinctus
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s10126-011-9408-1
关键词[WOS]	LUGWORM ARENICOLA-MARINA ; HYDROGEN-SULFIDE ; OXIDATIVE-PHOSPHORYLATION ; QUINONE OXIDOREDUCTASE ; SOLEMYA-REIDI ; INVERTEBRATE ; CAUPO ; MITOCHONDRIA ; ADAPTATIONS ; TOLERANCE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000305221200011
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6346
专题	资源植物与环境工程研究组
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Chonnam Natl Univ, Dept Aquaculture, Yeosu 550749, South Korea
推荐引用方式 GB/T 7714	Ma, Yu-Bin,Zhang, Zhi-Feng,Shao, Ming-Yu,et al. Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus[J]. MARINE BIOTECHNOLOGY,2012,14(2):245-251.
APA	Ma, Yu-Bin.,Zhang, Zhi-Feng.,Shao, Ming-Yu.,Kang, Kyoung-Ho.,Shi, Xiao-Li.,...&Li, Jin-Long.(2012).Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus.MARINE BIOTECHNOLOGY,14(2),245-251.
MLA	Ma, Yu-Bin,et al."Response of Sulfide:Quinone Oxidoreductase to Sulfide Exposure in the Echiuran Worm Urechis unicinctus".MARINE BIOTECHNOLOGY 14.2(2012):245-251.