Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain

doi:10.1016/j.biortech.2014.09.074

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	Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain
	Feng, Xinjun1,2 ; Ding, Yamei 3; Xian, Mo1 ; Xu, Xin1 ; Zhang, Rubing1 ; Zhao, Guang 1
	2014-11-01
发表期刊	BIORESOURCE TECHNOLOGY
卷号	172 页码:269-275
摘要	In this study, glycerol was used to produce optically pure D-lactate by engineered Klebsiella pneumoniae strain. In the recombinant strain, D-lactate dehydrogenase LdhA was overexpressed, and two genes, dhaT and yqhD for biosynthesis of main byproduct 1,3-propanediol, were knocked out. To further improve D-lactate production, the culture condition was optimized and the results demonstrated that aeration rate played an important role in D-lactate production. In microaerobic fed-batch fermentation, the engineered strain accumulated 142.1 g/L optically pure D-lactate with a yield of 0.82 g/g glycerol, which represented the highest D-lactate production from glycerol so far. This study showed that K. pneumoniae strain has high efficiency to convert glycerol into D-lactate and high potentiality in utilization of crude glycerol from biodiesel industry. (C) 2014 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	D-lactate Glycerol Engineered Klebsiella Pneumoniae Strain Bioconversion
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1016/j.biortech.2014.09.074
关键词[WOS]	D-LACTIC ACID ; RECOMBINANT ESCHERICHIA-COLI ; 3-HYDROXYPROPIONIC ACID ; MICROAEROBIC CONDITIONS ; CRUDE GLYCEROL ; 1,3-PROPANEDIOL ; DEHYDROGENASE ; FERMENTATION ; GENE ; METABOLISM
收录类别	SCI
语种	英语
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000344113800037
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6263
专题	生物基材料组群
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Feng, Xinjun,Ding, Yamei,Xian, Mo,et al. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain[J]. BIORESOURCE TECHNOLOGY,2014,172:269-275.
APA	Feng, Xinjun,Ding, Yamei,Xian, Mo,Xu, Xin,Zhang, Rubing,&Zhao, Guang.(2014).Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain.BIORESOURCE TECHNOLOGY,172,269-275.
MLA	Feng, Xinjun,et al."Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain".BIORESOURCE TECHNOLOGY 172(2014):269-275.