Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn(3)Cys(9) Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases | |
Zheng, Sanduo3,4; Wang, Jia3,4; Feng, Yingang1,2; Wang, Jinfeng2; Ye, Keqiong4 | |
2012-09-20 | |
发表期刊 | PLOS ONE |
卷号 | 7期号:9 |
摘要 | The dosage compensation complex (DCC) binds to single X chromosomes in Drosophila males and increases the transcription level of X-linked genes by approximately twofold. Male-specific lethal 2 (MSL2) together with MSL1 mediates the initial recruitment of the DCC to high-affinity sites in the X chromosome. MSL2 contains a DNA-binding cysteine-rich CXC domain that is important for X targeting. In this study, we determined the solution structure of MSL2 CXC domain by NMR spectroscopy. We identified three zinc ions in the CXC domain and determined the metal-to-cysteine connectivities from H-1-Cd-113 correlation experiments. The structure reveals an unusual zinc-cysteine cluster composed of three zinc ions coordinated by six terminal and three bridging cysteines. The CXC domain exhibits unexpected structural homology to pre-SET motifs of histone lysine methyltransferases, expanding the distribution and structural diversity of the CXC domain superfamily. Our findings provide novel structural insight into the evolution and function of CXC domains. |
文章类型 | Article |
WOS标题词 | Science & Technology |
DOI | 10.1371/journal.pone.0045437 |
关键词[WOS] | DOSAGE COMPENSATION COMPLEX ; MALE-SPECIFIC LETHAL-2 ; DNA-BINDING PROTEIN ; X-CHROMOSOME ; RING FINGER ; MACROMOLECULAR STRUCTURE ; DROSOPHILA-MELANOGASTER ; NMR STRUCTURE ; GENE ; METALLOTHIONEIN |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000309388900053 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6065 |
专题 | 代谢物组学研究组 |
作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao, Shangdong, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 3.Beijing Normal Univ, Dept Biochem & Mol Biol, Coll Life Sci, Beijing 100875, Peoples R China 4.Natl Inst Biol Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Sanduo,Wang, Jia,Feng, Yingang,et al. Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn(3)Cys(9) Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases[J]. PLOS ONE,2012,7(9). |
APA | Zheng, Sanduo,Wang, Jia,Feng, Yingang,Wang, Jinfeng,&Ye, Keqiong.(2012).Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn(3)Cys(9) Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases.PLOS ONE,7(9). |
MLA | Zheng, Sanduo,et al."Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn(3)Cys(9) Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases".PLOS ONE 7.9(2012). |
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