Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp Mutant Induced by Heavy Carbon Ions

doi:10.1371/journal.pone.0060700

QIBEBT-IR > 分子微生物工程研究组

	Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp Mutant Induced by Heavy Carbon Ions
	Hu, Guangrong 1; Fan, Yong 1; Zhang, Lei 1; Yuan, Cheng 1; Wang, Jufang 2; Li, Wenjian 2; Hu, Qiang 3; Li, Fuli1
	2013-04-09
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:4
摘要	The unicellular green microalga Desmodesmus sp. S1 can produce more than 50% total lipid of cell dry weight under high light and nitrogen-limitation conditions. After irradiation by heavy C-12(6+) ion beam of 10, 30, 60, 90 or 120 Gy, followed by screening of resulting mutants on 24-well microplates, more than 500 mutants were obtained. One of those, named D90G-19, exhibited lipid productivity of 0.298 g L-1 center dot d(-1), 20.6% higher than wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis under stress. This work demonstrated that heavy-ion irradiation combined with high-throughput screening is an effective means for trait improvement. The resulting mutant D90G-19 may be used for enhanced lipid production.
文章类型	Article
WOS标题词	Science & Technology
DOI	10.1371/journal.pone.0060700
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; CHLOROPHYLL FLUORESCENCE ; MICROALGAL BIOFUELS ; PHOTOSYSTEM-II ; RICE ; BACILLARIOPHYCEAE ; PHOTOINHIBITION ; BIOSYNTHESIS ; ARABIDOPSIS ; IRRADIATION
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
项目资助者	International cooperative project of Chinese Academy of Sciences(31010103907) ; "Western Light'' Talents Co-Scholar Program of Chinese Academy of Sciences(Y106140XBL)
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000317909600038
出版者	PUBLIC LIBRARY SCIENCE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6058
专题	分子微生物工程研究组
通讯作者	Li, Fuli
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 3.Arizona State Univ, Lab Algae Res & Biotechnol LARB, Coll Technol & Innovat, Mesa, AZ USA
推荐引用方式 GB/T 7714	Hu, Guangrong,Fan, Yong,Zhang, Lei,et al. Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp Mutant Induced by Heavy Carbon Ions[J]. PLOS ONE,2013,8(4).
APA	Hu, Guangrong.,Fan, Yong.,Zhang, Lei.,Yuan, Cheng.,Wang, Jufang.,...&Li, Fuli.(2013).Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp Mutant Induced by Heavy Carbon Ions.PLOS ONE,8(4).
MLA	Hu, Guangrong,et al."Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp Mutant Induced by Heavy Carbon Ions".PLOS ONE 8.4(2013).