Milligram Production and Biological Activity Characterization of the Human Chemokine Receptor CCR3 | |
Wang, Mingqing1,2; Ge, Baosheng1,2; Li, Renmin3; Wang, Xiaoqiang1,2; Lao, Jun1,2; Huang, Fang1,2 | |
2013-06-03 | |
发表期刊 | PLOS ONE |
卷号 | 8期号:6页码:1 |
摘要 | Human chemokine receptor CCR3 (hCCR3) belongs to the G protein-coupled receptors (GPCRs) superfamily of membrane proteins and plays major roles in allergic diseases and angiogenesis. In order to study the structural and functional mechanism of hCCR3, it is essential to produce pure protein with biological functions on a milligram scale. Here we report the expression of hCCR3 gene in a tetracycline-inducible stable mammalian cell line. A cell clone with high hCCR3 expression was selected from 46 stably transfected cell clones and from this cell line pure hCCR3 on a milligram scale was obtained after two-step purification. Circular dichroism spectrum with a characteristic shape and magnitude for alpha-helix indicated proper folding of hCCR3 after purification. The biological activity of purified hCCR3 was verified by its high binding affinity with its endogenous ligands CCL11 and CCL24, with K-D in the range of 10(-8) M to 10(-6) M. |
文章类型 | Article |
WOS标题词 | Science & Technology |
DOI | 10.1371/journal.pone.0065500 |
关键词[WOS] | PROTEIN-COUPLED RECEPTORS ; EOSINOPHIL EOTAXIN RECEPTOR ; HETEROLOGOUS EXPRESSION ; MOLECULAR-CLONING ; FUNCTIONAL EXPRESSION ; PURIFICATION ; SOLUBILIZATION ; GPCR ; TRAFFICKING ; RECRUITMENT |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000319872300084 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6034 |
专题 | 代谢物组学研究组 |
作者单位 | 1.China Univ Petr Huadong, State Key Lab Heavy Oil Proc, Qingdao, Shandong, Peoples R China 2.China Univ Petr Huadong, Ctr Bioengn & Biotechnol, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Mingqing,Ge, Baosheng,Li, Renmin,et al. Milligram Production and Biological Activity Characterization of the Human Chemokine Receptor CCR3[J]. PLOS ONE,2013,8(6):1. |
APA | Wang, Mingqing,Ge, Baosheng,Li, Renmin,Wang, Xiaoqiang,Lao, Jun,&Huang, Fang.(2013).Milligram Production and Biological Activity Characterization of the Human Chemokine Receptor CCR3.PLOS ONE,8(6),1. |
MLA | Wang, Mingqing,et al."Milligram Production and Biological Activity Characterization of the Human Chemokine Receptor CCR3".PLOS ONE 8.6(2013):1. |
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