RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically

doi:10.1039/c3mb70232d

QIBEBT-IR > 分子微生物工程研究组

	RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically
	Tan, Yang 1,2; Liu, Juanjuan 1; Chen, Xiaohua 1; Zheng, Huajun 3; Li, Fuli1
	2013
发表期刊	MOLECULAR BIOSYSTEMS
卷号	9 期号:11 页码:2775-2784
摘要	Clostridium ljungdahlii DSM 13528 represents a promising platform organism for production of a whole variety of different biofuels and biochemicals from syngas. Although the publication of its genome gave us the first possibility to understand the molecular mechanism for carbon utilization, reports on the profiling of the transcriptome were unavailable. In this study, RNA-seq-based global transcriptome analysis was performed to compare the transcriptomes of C. ljungdahlii grown on CO-CO2 with those grown on fructose. In total, 1852 differentially expressed genes were identified, which included 366 upregulated genes and 1486 downregulated genes under CO-CO2 conditions. These up- and down-regulated genes are predicted to be involved in the Wood-Ljungdahl pathway, CO2 reduction to acetic acid, fructose fermentation, central carbon metabolism and transport, and vitamin B12 synthesis. In addition, 36 small RNAs were identified, 20 of which were novel small RNAs. Quantitative real-time PCR (qRT-PCR) and RT-PCR analysis of the selected functional genes and sRNA genes expression profiles were found to be consistent with the RNA-seq data. The study allowed a deeper understanding of the molecular mechanisms underlying syngas utilization and could help guide the design of rational strategies to increase the efficiency of syngas fixation in the future.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1039/c3mb70232d
关键词[WOS]	SUBTILIS-TYRS GENE ; 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE ; NONCODING RNAS ; ANTITERMINATION ; GENOME ; IDENTIFICATION ; DEHYDROGENASE ; PURIFICATION ; DISCOVERY
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000325401800019
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/5957
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tan, Yang,Liu, Juanjuan,Chen, Xiaohua,et al. RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically[J]. MOLECULAR BIOSYSTEMS,2013,9(11):2775-2784.
APA	Tan, Yang,Liu, Juanjuan,Chen, Xiaohua,Zheng, Huajun,&Li, Fuli.(2013).RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically.MOLECULAR BIOSYSTEMS,9(11),2775-2784.
MLA	Tan, Yang,et al."RNA-seq-based comparative transcriptome analysis of the syngas-utilizing bacterium Clostridium ljungdahlii DSM 13528 grown autotrophically and heterotrophically".MOLECULAR BIOSYSTEMS 9.11(2013):2775-2784.