| Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol | |
Xu, Chenggang1 ; Qin, Yong1,2; Li, Yudong1,3; Ji, Yuetong1; Huang, Jianzhong2; Song, Houhui1; Xu, Jian1
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| 2010-12-01 | |
| 发表期刊 | BIORESOURCE TECHNOLOGY
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| 卷号 | 101期号:24页码:9560-9569 |
| 摘要 | The cellulosome, a multi-subunit protein complex catalyzing cellulose degradation in cellulolytic Clostridium thermocellum, plays a crucial role in Consolidated Bioprocessing (CBP) of lignocellulose into ethanol. Here, activity of cellulosome was tested under varying concentrations of chemical compounds derived from lignocellulose pretreatment and fermentation. We found that, firstly, the cellulolytic activity of cellulosome was actually promoted by formate, acetate and lactate; secondly, cellulosome was tolerant up to 5 mM furfural, 50 mM p-hydroxybenzoic acid and 1 mM catechol. Furthermore, the cellulosome exhibited higher ethanol tolerance and thermostability than commercialized fungal (Trichoderma reesei) cellulase. To probe the implication of these unique enzyme-features, C. thermocellum JYTO1 was cultured under conditions optimal for cellulosome activity. This CBP system yielded 491 mM ethanol, the highest level reported thus far for C. thermocellum monocultures. These findings demonstrate the potential advantages of bacterial cellulosome, and provide a novel strategy for design, selection and optimization of the cellulosome-ethanologen partnership.; The cellulosome, a multi-subunit protein complex catalyzing cellulose degradation in cellulolytic Clostridium thermocellum, plays a crucial role in Consolidated Bioprocessing (CBP) of lignocellulose into ethanol. Here, activity of cellulosome was tested under varying concentrations of chemical compounds derived from lignocellulose pretreatment and fermentation. We found that, firstly, the cellulolytic activity of cellulosome was actually promoted by formate, acetate and lactate; secondly, cellulosome was tolerant up to 5 mM furfural, 50 mM p-hydroxybenzoic acid and 1 mM catechol. Furthermore, the cellulosome exhibited higher ethanol tolerance and thermostability than commercialized fungal (Trichoderma reesei) cellulase. To probe the implication of these unique enzyme-features, C. thermocellum JYTO1 was cultured under conditions optimal for cellulosome activity. This CBP system yielded 491 mM ethanol, the highest level reported thus far for C. thermocellum monocultures. These findings demonstrate the potential advantages of bacterial cellulosome, and provide a novel strategy for design, selection and optimization of the cellulosome-ethanologen partnership. (C) 2010 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Cellulosome Fungal Cellulase Consolidated Bioprocessing Pretreatment Clostridium Thermocellum |
| 学科领域 | 功能基因组 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2010.07.065 |
| 关键词[WOS] | CLOSTRIDIUM-THERMOCELLUM ; TRICHODERMA-REESEI ; SACCHAROMYCES-CEREVISIAE ; WHEAT-STRAW ; POTENTIAL INHIBITORS ; CELLULASE PRODUCTION ; CONTINUOUS-CULTURE ; BETA-GLUCOSIDASE ; WET OXIDATION ; HIGH-YIELD |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000282201200021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/340 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, Qingdao 266101, Shandong, Peoples R China 2.Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Fuzhou 350108, Fujian, Peoples R China 3.Huaqiao Univ, Quanzhou 362021, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Chenggang,Qin, Yong,Li, Yudong,et al. Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol[J]. BIORESOURCE TECHNOLOGY,2010,101(24):9560-9569. |
| APA | Xu, Chenggang.,Qin, Yong.,Li, Yudong.,Ji, Yuetong.,Huang, Jianzhong.,...&Xu, Jian.(2010).Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol.BIORESOURCE TECHNOLOGY,101(24),9560-9569. |
| MLA | Xu, Chenggang,et al."Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol".BIORESOURCE TECHNOLOGY 101.24(2010):9560-9569. |
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