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Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol
Xu, Chenggang1; Qin, Yong1,2; Li, Yudong1,3; Ji, Yuetong1; Huang, Jianzhong2; Song, Houhui1; Xu, Jian1
2010-12-01
发表期刊BIORESOURCE TECHNOLOGY
卷号101期号:24页码:9560-9569
摘要The cellulosome, a multi-subunit protein complex catalyzing cellulose degradation in cellulolytic Clostridium thermocellum, plays a crucial role in Consolidated Bioprocessing (CBP) of lignocellulose into ethanol. Here, activity of cellulosome was tested under varying concentrations of chemical compounds derived from lignocellulose pretreatment and fermentation. We found that, firstly, the cellulolytic activity of cellulosome was actually promoted by formate, acetate and lactate; secondly, cellulosome was tolerant up to 5 mM furfural, 50 mM p-hydroxybenzoic acid and 1 mM catechol. Furthermore, the cellulosome exhibited higher ethanol tolerance and thermostability than commercialized fungal (Trichoderma reesei) cellulase. To probe the implication of these unique enzyme-features, C. thermocellum JYTO1 was cultured under conditions optimal for cellulosome activity. This CBP system yielded 491 mM ethanol, the highest level reported thus far for C. thermocellum monocultures. These findings demonstrate the potential advantages of bacterial cellulosome, and provide a novel strategy for design, selection and optimization of the cellulosome-ethanologen partnership.; The cellulosome, a multi-subunit protein complex catalyzing cellulose degradation in cellulolytic Clostridium thermocellum, plays a crucial role in Consolidated Bioprocessing (CBP) of lignocellulose into ethanol. Here, activity of cellulosome was tested under varying concentrations of chemical compounds derived from lignocellulose pretreatment and fermentation. We found that, firstly, the cellulolytic activity of cellulosome was actually promoted by formate, acetate and lactate; secondly, cellulosome was tolerant up to 5 mM furfural, 50 mM p-hydroxybenzoic acid and 1 mM catechol. Furthermore, the cellulosome exhibited higher ethanol tolerance and thermostability than commercialized fungal (Trichoderma reesei) cellulase. To probe the implication of these unique enzyme-features, C. thermocellum JYTO1 was cultured under conditions optimal for cellulosome activity. This CBP system yielded 491 mM ethanol, the highest level reported thus far for C. thermocellum monocultures. These findings demonstrate the potential advantages of bacterial cellulosome, and provide a novel strategy for design, selection and optimization of the cellulosome-ethanologen partnership. (C) 2010 Elsevier Ltd. All rights reserved.
文章类型Article
关键词Cellulosome Fungal Cellulase Consolidated Bioprocessing Pretreatment Clostridium Thermocellum
学科领域功能基因组
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2010.07.065
关键词[WOS]CLOSTRIDIUM-THERMOCELLUM ; TRICHODERMA-REESEI ; SACCHAROMYCES-CEREVISIAE ; WHEAT-STRAW ; POTENTIAL INHIBITORS ; CELLULASE PRODUCTION ; CONTINUOUS-CULTURE ; BETA-GLUCOSIDASE ; WET OXIDATION ; HIGH-YIELD
收录类别SCI
语种英语
WOS研究方向Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号WOS:000282201200021
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/340
专题单细胞中心组群
作者单位1.Chinese Acad Sci, Qingdao Inst BioEnergy & BioProc Technol, Qingdao 266101, Shandong, Peoples R China
2.Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Fuzhou 350108, Fujian, Peoples R China
3.Huaqiao Univ, Quanzhou 362021, Fujian, Peoples R China
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Xu, Chenggang,Qin, Yong,Li, Yudong,et al. Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol[J]. BIORESOURCE TECHNOLOGY,2010,101(24):9560-9569.
APA Xu, Chenggang.,Qin, Yong.,Li, Yudong.,Ji, Yuetong.,Huang, Jianzhong.,...&Xu, Jian.(2010).Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol.BIORESOURCE TECHNOLOGY,101(24),9560-9569.
MLA Xu, Chenggang,et al."Factors influencing cellulosome activity in Consolidated Bioprocessing of cellulosic ethanol".BIORESOURCE TECHNOLOGY 101.24(2010):9560-9569.
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