Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.

QIBEBT-IR > 资源植物与环境工程研究组

	Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.
	Chi, X.; Hu, R.; Yang, Q.; Zhang, X.; Pan, L.; Chen, N.; . . . Yu, S.
	2012
发表期刊	Mol Genet Genomics
卷号	287 期号:2 页码:167-176
摘要	Abstract Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Only a few studies on the reference genes have been done with peanut to date. In the present study, 14 potential reference genes in peanut were evaluated for their expression stability using the geNorm and NormFinder statistical algorithms. Expression stability was assessed by qRT-PCR across 32 biological samples, including various tissue types, seed developmental stages, salt and cold treatments. The results showed that the best-ranked references genes diVered across the samples. UKN1, UKN2, TUA5 and ACT11 were the most stable across all the tested samples. A combination of ACT11, TUA5, UKN2, PEPKR1 and TIP41 would be appropriate as a reference panel for normalizing gene expression data across the various tissues tested, whereas the combination of TUA5 and UKN1 was the most suitable for seed developmental stages. TUA5 and EF1b exhibited the most stable expression under cold treatment. For salt-treated leaves, TUA5 and UKN2 were the most stably expressed and HDC and UKN1 for salt-treated roots. The relative gene expression level of peanut Cys2/His2-type zinc Wnger protein gene AhZFP1 was analyzed in order to validate the reference genes selected for this study. These results provide guidelines for the selection of reference genes under diVerent experimental conditions and also a foundation for more accurate and widespread use of qRT-PCR in peanut gene analysis.
关键词	Peanut Qrt-pcr Reference Genes Validation
学科领域	能源植物资源
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1740
专题	资源植物与环境工程研究组
推荐引用方式 GB/T 7714	Chi, X.,Hu, R.,Yang, Q.,et al. Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.[J]. Mol Genet Genomics,2012,287(2):167-176.
APA	Chi, X..,Hu, R..,Yang, Q..,Zhang, X..,Pan, L..,...&. . . Yu, S..(2012).Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR..Mol Genet Genomics,287(2),167-176.
MLA	Chi, X.,et al."Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.".Mol Genet Genomics 287.2(2012):167-176.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Validation of refere（761KB）			开放获取	CC BY-NC-SA	浏览下载