| Metabolomics of photobiological hydrogen production induced by CCCP in Chlamydomonas reinhardtii | |
Yang, Dawei1,2,3; Zhang, Yanting1; Barupal, Dinesh Kumar2; Fan, Xiaolei1 ; Gustafson, Reid2; Guo, Rongbo1; Fiehn, Oliver2
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| 2014-01-02 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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| 卷号 | 39期号:1页码:150-158 |
| 摘要 | The green alga Chlamydomonas reinhardtii can produce hydrogen gas (H-2) in the presence of the proton uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The addition of 15 mu M CCCP to the algal cultures led to 13-fold increase in H-2 photoproduction compared to the control cultures without CCCP treatment. CCCP completely inhibited the photochemical activity of photosystem (PS) II under illumination. In order to better understand metabolic conditions necessary for sustained H-2 production, we have used gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF) for metabolomics analysis that is independent of nutritional stress, specifically, sulfur deprivation, which had been used previously to induce H-2 photoproduction. Even 10 min after addition of CCCP, metabolites from many metabolic modules were found drastically decreased, including levels of free amino acids, unsaturated free fatty acids and nucleotides. During prolonged CCCP exposure H-2 production was found to be stable for at least 12 h with a continued increase in levels of free fatty acids. These results indicate that CCCP might become a useful treatment for production of biohydrogen in reactors. The increase in fatty acid production might then be a useful addition for production of carbon-derived biofuels. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Solar Energy Large Scale Storage Renewable Energy Hydrogen Energy Energy Forecast |
| 学科领域 | 生物制氢与沼气 |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 关键词[WOS] | GREEN-ALGAE ; H-2 PRODUCTION ; NUTRIENT DEPRIVATION ; PHOTOPRODUCTION ; METABOLISM ; SUBCORDIFORMIS ; EVOLUTION ; DEPLETION ; PATHWAYS ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Electrochemistry ; Energy & Fuels |
| WOS类目 | Chemistry, Physical ; Electrochemistry ; Energy & Fuels |
| WOS记录号 | WOS:000330921800016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1729 |
| 专题 | 工业生物燃气研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.UC Davis Genome Ctr Metabol, Davis, CA 95616 USA 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Dawei,Zhang, Yanting,Barupal, Dinesh Kumar,et al. Metabolomics of photobiological hydrogen production induced by CCCP in Chlamydomonas reinhardtii[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2014,39(1):150-158. |
| APA | Yang, Dawei.,Zhang, Yanting.,Barupal, Dinesh Kumar.,Fan, Xiaolei.,Gustafson, Reid.,...&Fiehn, Oliver.(2014).Metabolomics of photobiological hydrogen production induced by CCCP in Chlamydomonas reinhardtii.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,39(1),150-158. |
| MLA | Yang, Dawei,et al."Metabolomics of photobiological hydrogen production induced by CCCP in Chlamydomonas reinhardtii".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 39.1(2014):150-158. |
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