| Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414 | |
Qi, Huan1,2,3; Bai, Fali1,2 ; Liu, Aihua1,2,3
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| 2013-04-01 | |
| 发表期刊 | BIOCHEMISTRY-MOSCOW
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| 卷号 | 78期号:4页码:424-430 |
| 摘要 | By combining anion-exchange chromatography with gel filtration, an effective method for purification of wild-type xyloglucanase and five other cellulolytic enzymes from strain QM9414 of Trichoderma reesei was established. Characterization by enzyme activity assay, SDS-PAGE, and mass spectrometry identified the purified proteins as cellobiohydrolases I and II, endoglucanases I and II, a xyloglucanase, and beta-xylosidase, of which the xyloglucanase was purified for the first time from the mutant strain QM9414. This method holds great promise to study the mechanism of cellulolytic enzymes, to investigate the synergistic action between cellulase and other cellulolytic enzymes, and to better exploit enzyme preparations for degradation of lignocellulose. |
| 文章类型 | Article |
| 关键词 | Xyloglucanase Cellulolytic Enzymes Purification Trichoderma Reesei Qm9414 |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1134/S0006297913040123 |
| 关键词[WOS] | BETA-GLUCOSIDASE ; THERMOSTABLE XYLANASES ; DEGRADING ENZYMES ; CELLULASE ; ENDOGLUCANASES ; XYLOSIDASE ; HYDROLYSIS ; CHROMATOGRAPHY ; FRACTIONATION ; QM-9414 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000317645000012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1690 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Huan,Bai, Fali,Liu, Aihua. Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414[J]. BIOCHEMISTRY-MOSCOW,2013,78(4):424-430. |
| APA | Qi, Huan,Bai, Fali,&Liu, Aihua.(2013).Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414.BIOCHEMISTRY-MOSCOW,78(4),424-430. |
| MLA | Qi, Huan,et al."Purification and characteristics of xyloglucanase and five other cellulolytic enzymes from Trichoderma reesei QM9414".BIOCHEMISTRY-MOSCOW 78.4(2013):424-430. |
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