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	Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116
	Gao, M.; Song, X.; Feng, Y.; Li, W.; & Cui, Q.
	2013
发表期刊	Journal of Oleo Science
卷号	62期号:3页码:143-151
摘要	A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to Aurantiochytrium species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28℃. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial fermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L－1, 71.09% of the DCW, and 17.42 g L－1 (34.79% of the TFA), respectively. The results show that Aurantiochytrium sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products.
关键词	Aurantiochytrium Sp. Biomass Docosahexaenoic Acid Heterotrophic Marine Algae Lipid
学科领域	代谢物组学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1675
专题	代谢物组学研究组
推荐引用方式 GB/T 7714	Gao, M.,Song, X.,Feng, Y.,et al. Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116[J]. Journal of Oleo Science,2013,62(3):143-151.
APA	Gao, M.,Song, X.,Feng, Y.,Li, W.,&& Cui, Q..(2013).Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116.Journal of Oleo Science,62(3),143-151.
MLA	Gao, M.,et al."Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116".Journal of Oleo Science 62.3(2013):143-151.

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