An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),

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	An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),
	Jinzhong Lin1; Jing Lu; Yingang Feng; Mengyi Sun; Keqiong Ye
	2013
发表期刊	PLoS biology
卷号	11 期号:10 页码:e1007669
摘要	A multitude of proteins and small nucleolar RNAs transiently associate with eukaryotic ribosomal RNAs to direct their modification and processing and the assembly of ribosomal proteins. Utp22 and Rrp7, two interacting proteins with no recognizable domain, are components of the 90S preribosome or the small subunit processome that conducts early processing of 18S rRNA. Here, we determine the cocrystal structure of Utp22 and Rrp7 complex at 1.97 A° resolution and the NMR structure of a C-terminal fragment of Rrp7, which is not visible in the crystal structure. The structure reveals that Utp22 surprisingly resembles a dimeric class I tRNA CCA-adding enzyme yet with degenerate active sites, raising an interesting evolutionary connection between tRNA and rRNA processing machineries. Rrp7 binds extensively to Utp22 using a deviant RNA recognition motif and an extended linker. Functional sites on the two proteins were identified by structure-based mutagenesis in yeast. We show that Rrp7 contains a flexible RNA-binding C-terminal tail that is essential for association with preribosomes. RNA–protein crosslinking shows that Rrp7 binds at the central domain of 18S rRNA and shares a neighborhood with two processing H/ACA snoRNAs snR30 and snR10. Depletion of snR30 prevents the stable assembly of Rrp7 into preribosomes. Our results provide insight into the evolutionary origin and functional context of Utp22 and Rrp7.
学科领域	代谢物组学
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1564
专题	代谢物组学研究组
推荐引用方式 GB/T 7714	Jinzhong Lin1,Jing Lu,Yingang Feng,et al. An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),[J]. PLoS biology,2013,11(10):e1007669.
APA	Jinzhong Lin1,Jing Lu,Yingang Feng,Mengyi Sun,&Keqiong Ye.(2013).An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),.PLoS biology,11(10),e1007669.
MLA	Jinzhong Lin1,et al."An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., 11(10),".PLoS biology 11.10(2013):e1007669.

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