| A Novel Dextran Dextrinase from Gluconobacter oxydans DSM-2003: Purification and Properties | |
Mao, Xiangzhao2; Wang, Shu1,4; Kan, Feifei2; Wei, Dongzhi3; Li, Fuli1
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| 2012-11-01 | |
| 发表期刊 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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| 卷号 | 168期号:5页码:1256-1264 |
| 摘要 | Dextran has already been widely applied in food, pharmaceutical, and chemical industries. In this study, a novel intracellular dextran dextrinase (DDase, EC 2.4.1.2) from Gluconobacter oxydans DSM-2003 exhibiting catalytic activity to synthesize dextran from maltodextrin was purified to homogeneity by ultrasonic cell disruption, ion exchange chromatography, and gel filtration. This procedure showed 187.5-fold purification from the cell-free extract with 41.9 % yield. And the apparent molecular weight was estimated to be 62 kDa by SDS-PAGE. It was different from the reported literatures, which found that the molecular weight of intracellular and extracellular DDase of G. oxydans ATCC-11894 was 300 and 152 kDa, respectively. Otherwise, it showed different physicochemical characteristics (optimal temperature and pH, thermal, pH stability, effect of metal ions) from the DDase of G. oxydans ATCC-11894. This indicated that DDase of G. oxydans DSM-2003 was a novel one compared to the reported literatures; Dextran has already been widely applied in food, pharmaceutical, and chemical industries. In this study, a novel intracellular dextran dextrinase (DDase, EC 2.4.1.2) from Gluconobacter oxydans DSM-2003 exhibiting catalytic activity to synthesize dextran from maltodextrin was purified to homogeneity by ultrasonic cell disruption, ion exchange chromatography, and gel filtration. This procedure showed 187.5-fold purification from the cell-free extract with 41.9 % yield. And the apparent molecular weight was estimated to be 62 kDa by SDS-PAGE. It was different from the reported literatures, which found that the molecular weight of intracellular and extracellular DDase of G. oxydans ATCC-11894 was 300 and 152 kDa, respectively. Otherwise, it showed different physicochemical characteristics (optimal temperature and pH, thermal, pH stability, effect of metal ions) from the DDase of G. oxydans ATCC-11894. This indicated that DDase of G. oxydans DSM-2003 was a novel one compared to the reported literatures. |
| 文章类型 | Article |
| 关键词 | Gluconobacter Oxydans Dextran Dextrinase Enzyme Purification Catalytic Property |
| 学科领域 | 微生物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12010-012-9854-x |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000311310300023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1557 |
| 专题 | 分子微生物工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Xiangzhao,Wang, Shu,Kan, Feifei,et al. A Novel Dextran Dextrinase from Gluconobacter oxydans DSM-2003: Purification and Properties[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,168(5):1256-1264. |
| APA | Mao, Xiangzhao,Wang, Shu,Kan, Feifei,Wei, Dongzhi,&Li, Fuli.(2012).A Novel Dextran Dextrinase from Gluconobacter oxydans DSM-2003: Purification and Properties.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,168(5),1256-1264. |
| MLA | Mao, Xiangzhao,et al."A Novel Dextran Dextrinase from Gluconobacter oxydans DSM-2003: Purification and Properties".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 168.5(2012):1256-1264. |
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| A Novel Dextran Dext（258KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
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