Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila

doi:10.1186/s13068-016-0494-1

QIBEBT-IR

	Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
	Ji,Shi-Qi; Wang,Bing; Lu,Ming; Li,Fu-Li
	2016-04-01
发表期刊	Biotechnology for Biofuels
ISSN	1754-6834
卷号	9 期号:1
摘要	AbstractBackgroundBrown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different oxidoreduction potentials to ethanol. Defluviitalea phaphyphila Alg1 is the first characterized thermophilic bacterium capable of direct utilization of brown algae.ResultsDefluviitalea phaphyphila Alg1 can simultaneously utilize mannitol, glucose, and alginate to produce ethanol, and high ethanol yields of 0.47?g/g-mannitol, 0.44?g/g-glucose, and 0.3?g/g-alginate were obtained. A rational redox balance system under obligate anaerobic condition in fermenting brown algae was revealed in D. phaphyphila Alg1 through genome and redox analysis. The excess reducing equivalents produced from mannitol metabolism were equilibrated by oxidizing forces from alginate assimilation. Furthermore, D. phaphyphila Alg1 can directly utilize unpretreated kelp powder, and 10?g/L of ethanol was accumulated within 72?h with an ethanol yield of 0.25?g/g-kelp. Microscopic observation further demonstrated the deconstruction process of brown algae cell by D. phaphyphila Alg1.ConclusionsThe integrated biomass deconstruction system of D. phaphyphila Alg1, as well as its high ethanol yield, provided us an excellent alternative for brown algae bioconversion at elevated temperature.
关键词	Brown algae Redox balance Bioethanol Marine thermophile Defluviitalea phaphyphila
DOI	10.1186/s13068-016-0494-1
语种	英语
WOS记录号	BMC:10.1186/s13068-016-0494-1
出版者	BioMed Central
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12617
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li,Fu-Li
作者单位	Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology
推荐引用方式 GB/T 7714	Ji,Shi-Qi,Wang,Bing,Lu,Ming,et al. Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila[J]. Biotechnology for Biofuels,2016,9(1).
APA	Ji,Shi-Qi,Wang,Bing,Lu,Ming,&Li,Fu-Li.(2016).Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila.Biotechnology for Biofuels,9(1).
MLA	Ji,Shi-Qi,et al."Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila".Biotechnology for Biofuels 9.1(2016).