QIBEBT-IR
Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system
Zhang,Jingjing1,2; Lu,Xuefeng1; Li,Jian-Jun1
2013-06-08
Source PublicationBiotechnology for Biofuels
ISSN1754-6834
Volume6Issue:1
AbstractAbstractBackgroundBiosynthesis of fatty alk(a/e)ne in cyanobacteria has been considered as a potential basis for the sunlight-driven and carbon-neutral bioprocess producing advanced solar biofuels. Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in that pathway. The heterologous or chemical reducing systems were generally used in in vitro ADO activity assay. The cognate electron transfer system from cyanobacteria to support ADO activity is still unknown.ResultsWe identified the potential endogenous reducing system including ferredoxin (Fd) and ferredoxin-NADP+ reductase (FNR) to support ADO activity in Synechococcus elongatus PCC7942. ADO (Synpcc7942_1593), FNR (SynPcc7942_0978), and Fd (SynPcc7942_1499) from PCC7942 were cloned, overexpressed, purified, and characterized. ADO activity was successfully supported with the endogenous electron transfer system, which worked more effectively than the heterologous and chemical ones. The results of the hybrid Fd/FNR reducing systems demonstrated that ADO was selective against Fd. And it was observed that the cognate reducing system produced less H2O2 than the heterologous one by 33% during ADO-catalyzed reactions. Importantly, kcat value of ADO 1593 using the homologous Fd/FNR electron transfer system is 3.7-fold higher than the chemical one.ConclusionsThe cognate electron transfer system from cyanobacteria to support ADO activity was identified and characterized. For the first time, ADO was functionally in vitro reconstituted with the endogenous reducing system from cyanobacteria, which supported greater activity than the surrogate and chemical ones, and produced less H2O2 than the heterologous one. The identified Fd/FNR electron transfer system will be potentially useful for improving ADO activity and further enhancing the biosynthetic efficiency of hydrocarbon biofuels in cyanobacteria.
KeywordBiofuels Fatty alk(a/e)ne Synechococcus elongatus PCC7942 Aldehyde-deformylating oxygenase Ferredoxin Ferredoxin-NADP+ reductase The cognate reducing system
DOI10.1186/1754-6834-6-86
Language英语
WOS IDBMC:10.1186/1754-6834-6-86
PublisherBioMed Central
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/12600
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorLu,Xuefeng; Li,Jian-Jun
Affiliation1.Chinese Academy of Sciences; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology
2.University of Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang,Jingjing,Lu,Xuefeng,Li,Jian-Jun. Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system[J]. Biotechnology for Biofuels,2013,6(1).
APA Zhang,Jingjing,Lu,Xuefeng,&Li,Jian-Jun.(2013).Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system.Biotechnology for Biofuels,6(1).
MLA Zhang,Jingjing,et al."Conversion of fatty aldehydes into alk (a/e)nes by in vitroreconstituted cyanobacterial aldehyde-deformylating oxygenase with the cognate electron transfer system".Biotechnology for Biofuels 6.1(2013).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang,Jingjing]'s Articles
[Lu,Xuefeng]'s Articles
[Li,Jian-Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang,Jingjing]'s Articles
[Lu,Xuefeng]'s Articles
[Li,Jian-Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang,Jingjing]'s Articles
[Lu,Xuefeng]'s Articles
[Li,Jian-Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.