Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
Yang,Jianming1; Nie,Qingjuan2; Ren,Meng3; Feng,Hongru4; Jiang,Xinglin1; Zheng,Yanning1; Liu,Min5; Zhang,Haibo1; Xian,Mo1
Source PublicationBiotechnology for Biofuels
AbstractAbstractBackgroundα-Pinene is an important natural product that is widely used in flavorings, fragrances, medicines, fine chemicals and high-density renewable fuels. Currently, α-Pinene used in industry is mainly produced either by tapping trees (gum turpentine) or as a byproduct of paper pulping (crude sulfate turpentine, CST). However, the extraction of it from trees is tedious and inefficient and requires substantial expenditure of natural resources. Therefore, it is necessary to seek sustainable technologies for α-pinene production.ResultsTo construct the microbial synthetic pathway of α-pinene in E. coli, we co-expressed native geranyl diphosphate synthase (IspA) from E. coli and α-pinene synthase (Pt30) from Pinus taeda, and then to increase the geranyl diphosphate (GPP) content in the cells, a suitable geranyl diphosphate synthase (GPPS2) was selected from two different origins. Furthermore, to enhance α-pinene production, a novel biosynthetic pathway of α-pinene was assembled in E. coli BL21(DE3) with the heterologous hybrid mevalonate (MVA) pathway, GPPS2 and α-pinene synthase (Pt30). The final genetic strain, YJM28, harboring the above novel biosynthetic pathway of α-pinene, accumulated α-pinene up to 5.44 mg/L and 0.97 g/L under flask and fed-batch fermentation conditions, respectively. The conversion efficiency of glucose to α-pinene (gram to gram) in the metabolically engineered strain reached 2.61%.ConclusionsIn this paper, by using metabolic engineering techniques, the more efficient biosynthetic pathway of α-pinene was successfully assembled in E. coli BL21(DE3) with the heterologous hybrid MVA pathway, GPPS2 and α-pinene synthase (Pt30). In addition, this is the first report on α-pinene fed-batch fermentation, and our results represent improvements over previous reports.
Keywordα-Pinene Geranyl diphosphate synthase α-Pinene Synthase E. coli
WOS IDBMC:10.1186/1754-6834-6-60
PublisherBioMed Central
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Document Type期刊论文
Corresponding AuthorZhang,Haibo; Xian,Mo
Affiliation1.Chinese Academy of Sciences; Qingdao Institute of Bioenergy and Bioprocess Technology
2.Qingdao Agricultural University; Foreign Languages School
3.Nanjing Agricultural University; School of Life and Science
4.Shenyang Agricultural University; College of Bioscience and Biotechnology
5.Wuhan University of Science and Technology; College of Chemical Engineering and Technology
Recommended Citation
GB/T 7714
Yang,Jianming,Nie,Qingjuan,Ren,Meng,et al. Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene[J]. Biotechnology for Biofuels,2013,6(1).
APA Yang,Jianming.,Nie,Qingjuan.,Ren,Meng.,Feng,Hongru.,Jiang,Xinglin.,...&Xian,Mo.(2013).Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene.Biotechnology for Biofuels,6(1).
MLA Yang,Jianming,et al."Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene".Biotechnology for Biofuels 6.1(2013).
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