Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene

doi:10.1186/1754-6834-6-60

QIBEBT-IR

	Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
	Yang,Jianming 1; Nie,Qingjuan 2; Ren,Meng 3; Feng,Hongru 4; Jiang,Xinglin 1; Zheng,Yanning 1; Liu,Min 5; Zhang,Haibo1 ; Xian,Mo1
	2013-04-30
发表期刊	Biotechnology for Biofuels
ISSN	1754-6834
卷号	6 期号:1
摘要	AbstractBackgroundα-Pinene is an important natural product that is widely used in flavorings, fragrances, medicines, fine chemicals and high-density renewable fuels. Currently, α-Pinene used in industry is mainly produced either by tapping trees (gum turpentine) or as a byproduct of paper pulping (crude sulfate turpentine, CST). However, the extraction of it from trees is tedious and inefficient and requires substantial expenditure of natural resources. Therefore, it is necessary to seek sustainable technologies for α-pinene production.ResultsTo construct the microbial synthetic pathway of α-pinene in E. coli, we co-expressed native geranyl diphosphate synthase (IspA) from E. coli and α-pinene synthase (Pt30) from Pinus taeda, and then to increase the geranyl diphosphate (GPP) content in the cells, a suitable geranyl diphosphate synthase (GPPS2) was selected from two different origins. Furthermore, to enhance α-pinene production, a novel biosynthetic pathway of α-pinene was assembled in E. coli BL21(DE3) with the heterologous hybrid mevalonate (MVA) pathway, GPPS2 and α-pinene synthase (Pt30). The final genetic strain, YJM28, harboring the above novel biosynthetic pathway of α-pinene, accumulated α-pinene up to 5.44 mg/L and 0.97 g/L under flask and fed-batch fermentation conditions, respectively. The conversion efficiency of glucose to α-pinene (gram to gram) in the metabolically engineered strain reached 2.61%.ConclusionsIn this paper, by using metabolic engineering techniques, the more efficient biosynthetic pathway of α-pinene was successfully assembled in E. coli BL21(DE3) with the heterologous hybrid MVA pathway, GPPS2 and α-pinene synthase (Pt30). In addition, this is the first report on α-pinene fed-batch fermentation, and our results represent improvements over previous reports.
关键词	α-Pinene Geranyl diphosphate synthase α-Pinene Synthase E. coli
DOI	10.1186/1754-6834-6-60
语种	英语
WOS记录号	BMC:10.1186/1754-6834-6-60
出版者	BioMed Central
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12587
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhang,Haibo; Xian,Mo
作者单位	1.Chinese Academy of Sciences; Qingdao Institute of Bioenergy and Bioprocess Technology 2.Qingdao Agricultural University; Foreign Languages School 3.Nanjing Agricultural University; School of Life and Science 4.Shenyang Agricultural University; College of Bioscience and Biotechnology 5.Wuhan University of Science and Technology; College of Chemical Engineering and Technology
推荐引用方式 GB/T 7714	Yang,Jianming,Nie,Qingjuan,Ren,Meng,et al. Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene[J]. Biotechnology for Biofuels,2013,6(1).
APA	Yang,Jianming.,Nie,Qingjuan.,Ren,Meng.,Feng,Hongru.,Jiang,Xinglin.,...&Xian,Mo.(2013).Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene.Biotechnology for Biofuels,6(1).
MLA	Yang,Jianming,et al."Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene".Biotechnology for Biofuels 6.1(2013).