Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction

doi:10.1186/1752-153X-7-165

QIBEBT-IR

	Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction
	Guo,Na 1; Liu,Mingtao 2; Yang,Dawei 3; Huang,Ying 1; Niu,Xiaohong 1; Wu,Ruifan 4; Liu,Ying 5; Ma,Guizhi 4; Dou,Deqiang 6
	2013-10-10
发表期刊	Chemistry Central Journal
ISSN	1752-153X
卷号	7 期号:1
摘要	AbstractBackgroundShen-Fu decoction is a traditional Chinese medicine prescription with a 3:2 ratio of Radix Ginseng and Fuzi (Radix Aconiti lateralis praeparata). Ginsenosides and alkaloids are considered to be the main active components of Shen-Fu decoction. However, no analytical methods have been used to quantitatively analyse both components in Shen-Fu decoction simultaneously.ResultsWe successfully developed a rapid resolution liquid chromatography coupled with tandem mass spectrometry (RRLC-MS/MS) method for the simultaneous analysis of seven ginsenosides and three aconitum alkaloids in Shen-Fu decoction, the decoction of Radix ginseng and Fuzi (Radix Aconiti lateralis praeparata). Chromatogrpahic separation by RPLC was achieved using a reversed-phase column and a water/acetonitrile mobile phase, containing 0.05% formic acid and using a gradient system. The method was optimized to allow for simultaneous analysis of all analytes in 11minutes without the need for baseline resolution of the components. Furthermore, the separation demonstrated good linearity (r > 0.9882), repeatability (RSD < 7.01%), intra- and inter-day precisions (RSD < 5.06%) and high yields of recovery (91.13-111.97%) for ten major constituents, namely ginsenoside-Re, Rg1, Rb1, Rc, Rb2, Rd, Rf, aconitine, hypacoitine and mesaconitine.ConclusionsThe developed method could be used as a rapid and reliable approach for assessment of the quantity of the major constituents in Shen-Fu decoction.
关键词	Ginsenosides Aconitum alkaloids Shen-Fu decoction RRLC-MS/MS
DOI	10.1186/1752-153X-7-165
语种	英语
WOS记录号	BMC:10.1186/1752-153X-7-165
出版者	Springer International Publishing
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12581
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Ma,Guizhi; Dou,Deqiang
作者单位	1.China Academy of Chinese Medical Sciences; Experimental Research Center 2.SRI International 3.Chinese Academy of Sciences; Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology 4.Xinjiang Medical University; College of Pharmacy 5.Chinese Academy of Medical Sciences and Peking Union Medical College; Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica 6.Liaoning University of Traditional Chinese Medicine; Department of Chinese Medicine Chemistry
推荐引用方式 GB/T 7714	Guo,Na,Liu,Mingtao,Yang,Dawei,et al. Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction[J]. Chemistry Central Journal,2013,7(1).
APA	Guo,Na.,Liu,Mingtao.,Yang,Dawei.,Huang,Ying.,Niu,Xiaohong.,...&Dou,Deqiang.(2013).Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction.Chemistry Central Journal,7(1).
MLA	Guo,Na,et al."Quantitative LC-MS/MS analysis of seven ginsenosides and three aconitumalkaloids in Shen-Fu decoction".Chemistry Central Journal 7.1(2013).