Microbial production of sabinene—a new terpene-based precursor of advanced biofuel

doi:10.1186/1475-2859-13-20

QIBEBT-IR

	Microbial production of sabinene—a new terpene-based precursor of advanced biofuel
	Zhang,Haibo1 ; Liu,Qiang 2; Cao,Yujin 1; Feng,Xinjun 1; Zheng,Yanning 1; Zou,Huibin 1; Liu,Hui 1; Yang,Jianming 1; Xian,Mo 1
	2014-02-10
发表期刊	Microbial Cell Factories
ISSN	1475-2859
卷号	13 期号:1
摘要	AbstractBackgroundSabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar.ResultsIn this study, sabinene was significantly produced by assembling a biosynthetic pathway using the methylerythritol 4-phosphate (MEP) or heterologous mevalonate (MVA) pathway combining the GPP and sabinene synthase genes in an engineered Escherichia coli strain. Subsequently, the culture medium and process conditions were optimized to enhance sabinene production with a maximum titer of 82.18?mg/L. Finally, the fed-batch fermentation of sabinene was evaluated using the optimized culture medium and process conditions, which reached a maximum concentration of 2.65?g/L with an average productivity of 0.018?g?h-1?g-1 dry cells, and the conversion efficiency of glycerol to sabinene (gram to gram) reached 3.49%.ConclusionsThis is the first report of microbial synthesis of sabinene using an engineered E. coli strain with the renewable carbon source as feedstock. Therefore, a green and sustainable production strategy has been established for sabinene.
关键词	Sabinene Geranyl diphosphate synthase Sabinene synthase Escherichia coli
DOI	10.1186/1475-2859-13-20
语种	英语
WOS记录号	BMC:10.1186/1475-2859-13-20
出版者	BioMed Central
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12578
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Yang,Jianming; Xian,Mo
作者单位	1.Chinese Academy of Sciences; CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology 2.Sichuan Agricultural University; College of Food Science
推荐引用方式 GB/T 7714	Zhang,Haibo,Liu,Qiang,Cao,Yujin,等. Microbial production of sabinene—a new terpene-based precursor of advanced biofuel[J]. Microbial Cell Factories,2014,13(1).
APA	Zhang,Haibo.,Liu,Qiang.,Cao,Yujin.,Feng,Xinjun.,Zheng,Yanning.,...&Xian,Mo.(2014).Microbial production of sabinene—a new terpene-based precursor of advanced biofuel.Microbial Cell Factories,13(1).
MLA	Zhang,Haibo,et al."Microbial production of sabinene—a new terpene-based precursor of advanced biofuel".Microbial Cell Factories 13.1(2014).