Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052

doi:10.1111/j.1472-765X.2012.03283.x

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	Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052
	Zhang, W. L.1,2; Liu, Z. Y.1; Liu, Z.1; Li, F. L.1
	2012-09-01
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
卷号	55 期号:3 页码:240-246
摘要	Aims: To determine whether corncob residue (CCR) could be a good substrate for butanol production. Methods and Results: In this study, Ca(OH)₂ detoxification technique was used to remove inhibitors of lignocellulose enzymatic hydrolysis. During fermentation of untreated corncob residue hydrolysate (CCRH) by Clostridium beijerinckii NCIMB 8052, cell growth was inhibited and only 3·8 g l⁻¹ acetone, butanol and ethanol (ABE) was produced. After pretreatment with Ca(OH)₂, enzymatic hydrolysis of CCR resulted in 49·3 g l⁻¹ total sugars, about twofold of that of untreated one. In the fermentation of the Ca(OH)₂-detoxified CCRH, sugar utilization ratio was increased by 27·3%. When using the Ca(OH)₂-detoxified CCRH supplemented with 10 g l⁻¹ glucose, 16·0 g l⁻¹ ABE was produced, resulting in an ABE yield of 0·32 and a productivity of 0·33 g l⁻¹ h⁻¹. Conclusion: The results in this study suggest that CCR was a good carbon source for ABE fermentation. Significance and Impact of the Study: It is the first time to use CCR as substrate for butanol production. Ca(OH)₂ detoxification pretreatment was proved to be an effective method to improve enzymatic digestibility of lignocellulose. ; Aims: To determine whether corncob residue (CCR) could be a good substrate for butanol production. Methods and Results: In this study, Ca(OH)2 detoxification technique was used to remove inhibitors of lignocellulose enzymatic hydrolysis. During fermentation of untreated corncob residue hydrolysate (CCRH) by Clostridium beijerinckii NCIMB 8052, cell growth was inhibited and only 3.8 g l-1 acetone, butanol and ethanol (ABE) was produced. After pretreatment with Ca(OH)2, enzymatic hydrolysis of CCR resulted in 49.3 g l-1 total sugars, about twofold of that of untreated one. In the fermentation of the Ca(OH)2-detoxified CCRH, sugar utilization ratio was increased by 27.3%. When using the Ca(OH)2-detoxified CCRH supplemented with 10 g l-1 glucose, 16.0 g l-1 ABE was produced, resulting in an ABE yield of 0.32 and a productivity of 0.33 g l-1 h-1. Conclusion: The results in this study suggest that CCR was a good carbon source for ABE fermentation. Significance and Impact of the Study: It is the first time to use CCR as substrate for butanol production. Ca(OH)2 detoxification pretreatment was proved to be an effective method to improve enzymatic digestibility of lignocellulose.
文章类型	Article
关键词	Butanol Ca(Oh)2 detoxifiCation Clostridium Beijerinckii Ncimb 8052 Corncob Residue
学科领域	微生物资源
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1111/j.1472-765X.2012.03283.x
关键词[WOS]	CHEMICAL PRETREATMENT METHODS ; ETHANOL-PRODUCTION ; CELLULASE PRODUCTION ; LIME PRETREATMENT ; FERMENTATION ; DETOXIFICATION ; ACID ; BAGASSE ; BA101 ; LIGNOCELLULOSE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000307719200010
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/1237
专题	分子微生物工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, W. L.,Liu, Z. Y.,Liu, Z.,et al. Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052[J]. LETTERS IN APPLIED MICROBIOLOGY,2012,55(3):240-246.
APA	Zhang, W. L.,Liu, Z. Y.,Liu, Z.,&Li, F. L..(2012).Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052.LETTERS IN APPLIED MICROBIOLOGY,55(3),240-246.
MLA	Zhang, W. L.,et al."Butanol production from corncob residue using Clostridium beijerinckii NCIMB 8052".LETTERS IN APPLIED MICROBIOLOGY 55.3(2012):240-246.

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