Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules

doi:10.1142/S108842461850061X

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	Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules
	Chen, Zhifeng 1,2,3; Chen, Jie 2,3,4; Ma, Nana 2,3,4; Zho, Haifeng 1; Cong, Zhiqi 2,3
	2018-09-01
发表期刊	JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
ISSN	1088-4246
卷号	22 期号:9-10 页码:831-836
摘要	We herein report the H2O2-dependent selective hydroxylation of naphthalene catalyzed by engineered P450BM3 with the assistance of dual-functional small molecules (DFSMs). The mutation at position 268 significantly improved the hydroxylation activity of P450BM3, which is quite different from those engineered P450BM3 peroxygenases and NADPH-dependent P450BM3 mutants previously reported, implicating the unique role of the residue at position 268. This study provides a potential approach to develop the practical hydroxylation biocatalyst of P450s for aromatic hydrocarbons using the DFSM-facilitated P450BM3-H2O2 system.
关键词	P450 peroxygenase hydrogen peroxide hydroxylation naphthalene dual-functional small molecules
DOI	10.1142/S108842461850061X
关键词[WOS]	CYTOCHROME P450BM-3 ; ACTIVATION ; EVOLUTION ; H2O2
语种	英语
资助项目	NSFC[21778060] ; Shandong Provincial Key Laboratory of Synthetic Biology[SPKLSB-2017-02] ; Qingdao Innovative Leading Talent Project
WOS研究方向	Chemistry
项目资助者	NSFC ; Shandong Provincial Key Laboratory of Synthetic Biology ; Qingdao Innovative Leading Talent Project
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000442266200017
出版者	WORLD SCI PUBL CO INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12206
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zho, Haifeng; Cong, Zhiqi
作者单位	1.China Three Gorges Univ, Coll Biol & Pharmaceut Sci, Hubei Key Lab Nat Prod Res & Dev, Yichang 443002, Hubei, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Zhifeng,Chen, Jie,Ma, Nana,et al. Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules[J]. JOURNAL OF PORPHYRINS AND PHTHALOCYANINES,2018,22(9-10):831-836.
APA	Chen, Zhifeng,Chen, Jie,Ma, Nana,Zho, Haifeng,&Cong, Zhiqi.(2018).Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules.JOURNAL OF PORPHYRINS AND PHTHALOCYANINES,22(9-10),831-836.
MLA	Chen, Zhifeng,et al."Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules".JOURNAL OF PORPHYRINS AND PHTHALOCYANINES 22.9-10(2018):831-836.