| Phagemid Vectors for Phage Display: Properties, Characteristics and Construction | |
| Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2 | |
| 2012-03-30 | |
| 发表期刊 | JOURNAL OF MOLECULAR BIOLOGY
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| 卷号 | 417期号:3页码:129-143 |
| 摘要 | Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.; Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved. |
| 文章类型 | Review |
| 关键词 | Phage Display Phagemid Filamentous Phage Phagemid Vector Construction |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.jmb.2012.01.038 |
| 关键词[WOS] | COMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000301805800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1210 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China 4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143. |
| APA | Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,Petrenko, Valery,&Liu, Aihua.(2012).Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.JOURNAL OF MOLECULAR BIOLOGY,417(3),129-143. |
| MLA | Qi, Huan,et al."Phagemid Vectors for Phage Display: Properties, Characteristics and Construction".JOURNAL OF MOLECULAR BIOLOGY 417.3(2012):129-143. |
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