QIBEBT-IR  > 生物传感技术团队
Phagemid Vectors for Phage Display: Properties, Characteristics and Construction
Qi, Huan2; Lu, Haiqin3; Qiu, Hua-Ji4; Petrenko, Valery1; Liu, Aihua2
2012-03-30
Source PublicationJOURNAL OF MOLECULAR BIOLOGY
Volume417Issue:3Pages:129-143
AbstractPhagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.; Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted. (C) 2012 Elsevier Ltd. All rights reserved.
SubtypeReview
KeywordPhage Display Phagemid Filamentous Phage Phagemid Vector Construction
Subject Area生物传感器技术
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jmb.2012.01.038
WOS KeywordCOMBINATORIAL ANTIBODY LIBRARIES ; IN-VIVO RECOMBINATION ; STRANDED PLASMID DNA ; FILAMENTOUS PHAGE ; FAB FRAGMENTS ; BINDING-PROTEINS ; CLONING VEHICLE ; PEPTIDE LIBRARY ; HELPER PHAGE ; SELECTION
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000301805800001
Citation statistics
Cited Times:57[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1210
Collection生物传感技术团队
Affiliation1.Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
3.Southeast Univ, Sch Med, Nanjing 210009, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
Recommended Citation
GB/T 7714
Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,et al. Phagemid Vectors for Phage Display: Properties, Characteristics and Construction[J]. JOURNAL OF MOLECULAR BIOLOGY,2012,417(3):129-143.
APA Qi, Huan,Lu, Haiqin,Qiu, Hua-Ji,Petrenko, Valery,&Liu, Aihua.(2012).Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.JOURNAL OF MOLECULAR BIOLOGY,417(3),129-143.
MLA Qi, Huan,et al."Phagemid Vectors for Phage Display: Properties, Characteristics and Construction".JOURNAL OF MOLECULAR BIOLOGY 417.3(2012):129-143.
Files in This Item: Download All
File Name/Size DocType Version Access License
Phagemid vectors for(975KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qi, Huan]'s Articles
[Lu, Haiqin]'s Articles
[Qiu, Hua-Ji]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qi, Huan]'s Articles
[Lu, Haiqin]'s Articles
[Qiu, Hua-Ji]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qi, Huan]'s Articles
[Lu, Haiqin]'s Articles
[Qiu, Hua-Ji]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Phagemid vectors for phage display properties, characteristics and construction..pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.